Anti-IFIT1 抗体 [OTI3G8] (ab118062)

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Western blot - Anti-IFIT1 antibody [OTI3G8] (ab118062)

Key features and details

Mouse monoclonal [OTI3G8] to IFIT1
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Human, African green monkey
Isotype: IgG1

製品名

Anti-IFIT1 antibody [OTI3G8]
IFIT1 一次抗体製品一覧
製品の詳細

Mouse monoclonal [OTI3G8] to IFIT1
由来種

Mouse
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
種交差性

交差種: Human, African green monkey
免疫原

Recombinant full length protein corresponding to Human IFIT1. Recombinant full length Human IFIT1 produced in HEK293T cells (NP_000899).
Database link: P09914
ポジティブ･コントロール
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY IFIT1 cDNA. IHC-P: Human liver carcinoma, Human ovary adenocarcinoma, kidney and liver tissues. ICC/IF: HT29 cells and COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 cDNA. Flow Cyt (Intra): HEK293T cells transfected with pCMV6-ENTRY IFIT1 overexpress plasmid
特記事項

The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. The product can be stored for up to 12 months.
バッファー

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 48% PBS, 1% BSA
Concentration information loading...
精製度

IgG fraction
特記事項（精製）

ab118062 is purified from TCS by affinity chromatography.
ポリ/モノ

モノクローナル
クローン名

OTI3G8
アイソタイプ

IgG1
研究分野

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Positive Controls
- Jurkat whole cell lysate (ab7899)
Recombinant Protein
- Recombinant Human IFIT1 protein (ab152468)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab118062の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB		1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P		1/50.
ICC/IF		1/50 - 1/100.

特記事項
Flow Cyt (Intra) 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/50.
ICC/IF 1/50 - 1/100.

配列類似性

Belongs to the IFIT family.
Contains 10 TPR repeats.
Target information above from: UniProt accession P09914 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 3434 Human
- Omim: 147690 Human
- SwissProt: P09914 Human
- Unigene: 20315 Human
別名
- C56 antibody
- G10P1 antibody
- GARG 16 antibody
- GARG16 antibody
- IFI 56 antibody
- IFI 56K antibody
- IFI-56K antibody
- IFI56 antibody
- IFIT-1 antibody
- Ifit1 antibody
- IFIT1_HUMAN antibody
- IFNAI1 antibody
- Interferon alpha inducible protein (MW 56kD) antibody
- Interferon induced 56 kDa protein antibody
- Interferon induced protein 56 antibody
- Interferon inducible mRNA 561 antibody
- Interferon-induced 56 kDa protein antibody
- Interferon-induced protein with tetratricopeptide repeats 1 antibody
- ISG56 antibody
- OTTHUMP00000020062 antibody
- P56 antibody
- RNM561 antibody
see all

Western blot - Anti-IFIT1 antibody [OTI3G8] (ab118062)

All lanes : Anti-IFIT1 antibody [OTI3G8] (ab118062) at 1/500 dilution

Lane 1 : HEK293T cell lyaste, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY IFIT1 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 55 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203320)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in HT29 cells by Immunofluorescence.
Flow Cytometry (Intracellular) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/100 dilution staining IFIT1 in HEK293T cells transfected with either pCMV6-ENTRY IFIT1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry (Intracellular).

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab118062 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab118062 は 2 報の論文で使用されています。

Gao Y et al. Fusobacterium nucleatum stimulates cell proliferation and promotes PD-L1 expression via IFIT1-related signal in colorectal cancer. Neoplasia 35:100850 (2023). PubMed: 36371909
Berard AR et al. Comparative proteomic analyses of two reovirus T3D subtypes and comparison to T1L identifies multiple novel proteins in key cellular pathogenic pathways. Proteomics 15:2113-35 (2015). PubMed: 25900405

レビューと Q&A

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Question

Staining of dog cells was non-specific by ICC and also flow cytometry.

Abcam community

Verified customer

Asked on Oct 12 2012

Answer

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service.

Abcam Scientific Support

Answered on Oct 12 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

特記事項（精製）

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

配列類似性

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (2)

レビューと Q&A