AB118062

Anti-IFIT1 抗体 [OTI3G8]

Name: Anti-IFIT1 抗体 [OTI3G8] (ab118062) | Abcam
Brand: Abcam Limited
SKU: AB118062
Price: 71000 JPY
Availability: InStock

Anti-IFIT1 antibody [OTI3G8]

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(4 Publications)

Mouse Monoclonal IFIT1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Human, African green monkey, Transfected cell line - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFIT1.

別名を表示する

G10P1, IFI56, IFNAI1, ISG56, IFIT1, Antiviral innate immune response effector IFIT1, IFIT-1, Interferon-induced 56 kDa protein, Interferon-induced protein with tetratricopeptide repeats 1, IFI-56K, P56

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (AB118062)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (AB118062)

ab118062 at 1/50 dilution staining IFIT1 in HT29 cells by Immunofluorescence.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry.

Flow Cytometry (Intracellular) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

ab118062 at 1/100 dilution staining IFIT1 in HEK293T cells transfected with either pCMV6-ENTRY IFIT1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry (Intracellular).

Unknown

Western blot - Anti-IFIT1 antibody [OTI3G8] (AB118062)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203320)

All lanes:

Western blot - Anti-IFIT1 antibody [OTI3G8] (ab118062) at 1/500 dilution

Lane 1:

HEK293T cell lyaste, transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T cell lysate, transfected with pCMV6-ENTRY IFIT1 cDNA at 5 µg

Predicted band size: 55 kDa

false

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (AB118062)

ab118062 at 1/50 dilution staining IFIT1 in COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 by Immunofluorescence.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

OTI3G8

アイソタイプ

IgG1

キャリアフリー

交差種

Human, African green monkey

アプリケーション

WB, IHC-P, ICC/IF, Flow Cyt (Intra)

applications

免疫原

Recombinant Full Length Protein corresponding to Human IFIT1.

P09914

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

精製に関する特記事項

ab118062 is purified from TCS by affinity chromatography.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|The product can be stored for up to 12 months

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

IFIT1 also known as Interferon-Induced Protein with Tetratricopeptide Repeats 1 is a protein that plays a significant role in antiviral defense mechanisms. It has a molecular mass of approximately 56 kDa. Researchers find IFIT1 predominantly expressed in cells stimulated by interferons. The protein detects viral single-stranded RNA which in turn helps initiate the antiviral response. You will find IFIT1 in various cell types mainly those exposed to viral infections as it is an important part of the body's innate immune system.

Biological function summary

Activities of IFIT1 focus on inhibiting viral replication and translation processes. The protein binds to viral RNAs via its distinctive sensor domains preventing infections from progressing. IFIT1 operates often as part of a larger protein complex with other IFIT family members improving its ability to intercept and inhibit viral RNA. This concerted action provides an important line of defense against diverse viral pathogens by interfering with viral protein synthesis.

Pathways

IFIT1 occupies an important place in the interferon signaling pathways particularly the Jak-STAT pathway. This pathway is critical for mounting antiviral responses when viral infections occur. Through its interactions within the pathway IFIT1 works together with related proteins like IFIT2 and IFIT3 to repel viral threats. By integrating into these pathways it contributes to amplifying the immune response promoting a robust defense system against foreign viral invaders.

IFIT1 is associated mainly with viral diseases such as influenza and hepatitis C. Its role in these diseases involves preventing the communication and replication of the viruses making it a pivotal part of antiviral therapeutic research. Additionally proteins like RIG-I interact with IFIT1 during these processes to recognize viral RNA enhancing the effectiveness of the immune response. These protein partnerships drive ongoing research looking to leverage IFIT1's antiviral properties for treatment developments.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a key role in the innate immune response as part of an interferon-dependent multiprotein complex, recognizing and sequestering viral RNAs that lack host-specific 2'-O-methylation at their 5' cap. By distinguishing these RNAs from host mRNAs, inhibits their translation by competing with the translation initiation factor eIF4E (PubMed : 21642987, PubMed : 27240734, PubMed : 39009378, PubMed : 23334420, PubMed : 28251928, PubMed : 36285486). Could also prevent viral replication through its interaction with DNA replication origin-binding protein E1 of several viruses. Causes the translocation of E1 from the nucleus to the cytoplasm and can also inhibit its helicase activity in vitro (PubMed : 19008854, PubMed : 21976647). Exhibits antiviral activity against many viruses from the Flaviviridae (West Nile virus, Dengue virus, hepatitis C virus), Coronaviridae (human 229E coronavirus, SARS-CoV-2 and SARS-CoV), Poxviridae (vaccinia virus) and Togaviridae (Sindbis virus) families (PubMed : 19008854, PubMed : 21976647, PubMed : 28251928, PubMed : 36285486).

See full target information IFIT1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Neoplasia (New York, N.Y.) 35:100850 PubMed36371909

2022

Fusobacterium nucleatum stimulates cell proliferation and promotes PD-L1 expression via IFIT1-related signal in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Gao,Tianhui Zou,Pingping Xu,Yingchao Wang,Yi Jiang,Ying-Xuan Chen,Haoyan Chen,Jie Hong,Jing-Yuan Fang

Acta physiologica (Oxford, England) 215:89-104 PubMed26235702

2015

Mouse white adipose tissue-derived mesenchymal stem cells gain pericentral and periportal hepatocyte features after differentiation in vitro, which are preserved in vivo after hepatic transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

S Winkler,M Hempel,S Brückner,F Mallek,A Weise,T Liehr,H-M Tautenhahn,M Bartels,B Christ

Proteomics 15:2113-35 PubMed25900405

2015

Comparative proteomic analyses of two reovirus T3D subtypes and comparison to T1L identifies multiple novel proteins in key cellular pathogenic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia R Berard,Alberto Severini,Kevin M Coombs

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IFIT1 抗体 [OTI3G8]

Anti-IFIT1 antibody [OTI3G8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Flow Cytometry (Intracellular) - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Western blot - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (AB118062)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise