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リコンビナントRabMAb

Anti-IER3IP1 抗体 [EPR13440] (ab181247)

Submit a review Submit a question References (1)
Western blot - Anti-IER3IP1 antibody [EPR13440] (ab181247)
  • Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] (ab181247)
  • Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] (ab181247)
  • Flow Cytometry (Intracellular) - Anti-IER3IP1 antibody [EPR13440] (ab181247)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)
  • Anti-IER3IP1 antibody [EPR13440] (ab181247)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13440] to IER3IP1
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-IER3IP1 antibody [EPR13440]
    IER3IP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13440] to IER3IP1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, HeLa and Human fetal heart lysates; Human kidney and Human cardiac muscle tissues; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13440

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181247の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
ICC/IF
1/100 - 1/250.
IP
1/50 - 1/70.
特記事項
Flow Cyt (Intra)
1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
ICC/IF
1/100 - 1/250.
IP
1/50 - 1/70.

ターゲット情報

画像

  • Western blot - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Western blot - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    All lanes : Anti-IER3IP1 antibody [EPR13440] (ab181247) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : HeLa lysate
    Lane 3 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 9 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling IER3IP1 with ab181247 at 1/250 dilution, dollowed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and DAPI staining (blue).

  • Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Western blot analysis of immunoprecipitation pellet from HeLa cell lysate  immunoprecipitated using ab181247 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000.

  • Flow Cytometry (Intracellular) - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Flow Cytometry (Intracellular) - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Intracellular flow cytometricl analysis of HeLa cells (paraformaldehyde-fixed, 2%)labeling IER3IP1 with ab181247 at 1/210 (red) compared to a negative control antibody (green),followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Anti-IER3IP1 antibody [EPR13440] (ab181247)

参考文献 (1)

ab181247 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181247 は 1 報の論文で使用されています。

  • Mao MH  et al. Additive antitumor effect of arsenic trioxide combined with intravesical bacillus Calmette-Guerin immunotherapy against bladder cancer through blockade of the IER3/Nrf2 pathway. Biomed Pharmacother 107:1093-1103 (2018). PubMed: 30257321

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