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AB316988

Anti-Iduronate 2 sulfatase/SIDS 抗体 [EPR25954-155] - BSA and Azide free (Detector)

Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-155] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Iduronate 2 sulfatase/SIDS antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

別名を表示する

SIDS, IDS, Iduronate 2-sulfatase, Alpha-L-iduronate sulfate sulfatase, Idursulfase

1 Images
Sandwich ELISA - Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-155] - BSA and Azide free (Detector) (AB316988)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Iduronate 2 sulfatase/SIDS antibody [EPR25954-155] - BSA and Azide free (Detector) (AB316988)

Sandwich ELISA of ab316986 with the capture antibody ab316987 dilution at 2 ug/mL and detector antibody ab316988 dilution at 0.5 ug/mL.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25954-155

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab316988 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Iduronate 2 sulfatase/SIDS. The recommended pair for sandwich ELISA is:

Capture: ab316987, Human Iduronate 2 sulfatase/SIDS Capture Antibody (unconjugated)

Detector: ab316988, Human Iduronate 2 sulfatase/SIDS Detector Antibody (unconjugated)

The reference range value is 31.25-2000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Iduronate 2-sulfatase also known as IDS or iduronate 2-sulfatase protein is an enzyme responsible for hydrolyzing the 2-sulfate groups from the sugar iduronate in glycosaminoglycans (GAGs) such as dermatan sulfate and heparan sulfate. This action is necessary for the lysosomal degradation of GAGs. The IDS protein has a molecular mass of approximately 76 kDa and predominantly resides in lysosomes. It is widely expressed across human tissues but shows higher expression levels in cells involved with significant lysosomal activities.
Biological function summary

IDS functions to ensure proper breakdown and recycling of GAGs preventing their excessive accumulation. Although IDS acts independently its proper functioning complements other enzymes within the lysosomal degradation system. This lysosomal catabolic activity helps maintain cellular homeostasis as GAGs need controlled degradation for effective cell and tissue functions.

Pathways

IDS plays an important role in the lysosomal degradation pathway. By participating in the catabolism of GAGs IDS works alongside other enzymes such as alpha-L-iduronidase. This interaction is part of the larger lysosomal storage and cellular turnover pathways. These pathways are essential for cellular regulation and help prevent the buildup of undegraded molecules that can disrupt cellular functions.

Faulty IDS activity leads to mucopolysaccharidosis type II also known as Hunter syndrome. This disorder arises from defective GAG breakdown resulting in their accumulation causing various systemic symptoms. IDS is closely related to other lysosomal storage disorder proteins like arylsulfatase B as both enzymes collaborate in GAG degradation. Proper comprehension of IDS and its functioning aids in understanding therapeutic approaches to managing associated disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.
See full target information IDS
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組合せ製品

Assay Kits

AB289842

Total Glycosaminoglycans Assay Kit (Colorimetric)

Functional Studies - Total Glycosaminoglycans Assay Kit (Colorimetric) (AB289842)

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Cell Lines & Lysates

AB264747

Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell line

Sanger Sequencing - Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell line (AB264747)

  • 0
  • 0
Cell Lines & Lysates

AB258462

Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell lysate

Sanger Sequencing - Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell lysate (AB258462)

  • 0
  • 0

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