AB205617

Anti-IDI1 + IDI2 抗体 [EPR17213]

Anti-IDI1 + IDI2 antibody [EPR17213]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal IDI1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

別名を表示する

Isopentenyl-diphosphate Delta-isomerase 1, Isopentenyl pyrophosphate isomerase 1, IPP isomerase 1, IPPI1, IDI1, Isopentenyl-diphosphate delta-isomerase 2, Isopentenyl pyrophosphate isomerase 2, IPP isomerase 2, IPPI2, IDI2

4 Images

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Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Blocking and diluting buffer was 5% NFDM /TBST.

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Blocking and diluting buffer was 5% NFDM /TBST

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal skeletal muscle lysate at 10 µg

Lane 3:

Human colon cancer lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Unknown

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Blocking and diluting buffer was 5% NFDM /TBST.

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with IDI1 peptide

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with IDI2 peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Blocking and diluting buffer was 5% NFDM /TBST

Exposure time Lane 1 and 2 : 2minutes;
Lane 3 : 3minutes

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 2min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17213

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The isopentenyl-diphosphate delta isomerase 1 also known as IDI1 and its paralog IDI2 are enzymes that play an important role in isoprenoid biosynthesis. IDI1 has a molecular weight of approximately 27 kDa. Both IDI1 and IDI2 facilitate the interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) two critical intermediates in the mevalonate pathway. These enzymes express widely across different human tissues with notable expression in the liver brain and skeletal muscle.

Biological function summary

IDI1 and IDI2 contribute to the regulation of cholesterol and other sterol biosynthesis by converting linear five-carbon units into branched forms necessary for further pathway reactions. Although not usually part of a larger complex these enzymes directly impact the supply of precursors for the synthesis of essential biomolecules such as steroids dolichols and ubiquinones. This transformation is not only pivotal for maintaining lipid homeostasis but also for facilitating cellular proliferation and differentiation.

Pathways

IDI1 and IDI2 should be considered during discussions on the mevalonate pathway which is an important component of cholesterol synthesis and other essential biomolecule formation pathways. These enzymes interact with other proteins involved in the mevalonate pathway including HMG-CoA reductase which functions upstream of IPP and DMAPP isomerization. IDI1 and IDI2 function vitally to ensure a balance between IPP and DMAPP affecting the synthesis rates of downstream isoprenoids and sterols essential for cell membrane structure and signaling.

Dysregulation of IDI1 and IDI2 has connections to metabolic disorders such as dyslipidemia and certain types of cancer. Altered expression or mutation in these enzymes could disrupt normal cholesterol synthesis potentially contributing to high lipid levels in the blood as seen in dyslipidemia. Furthermore IDI1 connects to oncogenesis possibly contributing to cancer development by aiding in producing isoprenoid derivatives critical for tumor cell proliferation. In these situations the enzymes interact closely with proteins regulating cell growth like Ras through their role in providing necessary lipid modifiers for membrane anchoring and function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).

See full target information IDI1

追加のターゲット

Isopentenyl-diphosphate delta-isomerase 2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular biomedicine 6:78 PubMed41071519

2025

Lipin3 deficiency promotes hepatocyte ferroptosis and pyroptosis via activating JAK1-STAT3 pathway during acetaminophen induced acute liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Xing Liu,Qian Wang,Zi-Yu Xiangyang,Jie-Yi Long,Hao Huang,Liang-Liang Fan

Cell death & disease 15:704 PubMed39349433

2024

Cholesterol inhibition enhances antitumor response of gilteritinib in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Yue Sun,Di Cao,Yue-Ning Wang,Nuo-Qing Weng,Qian-Nan Ren,Shuo-Cheng Wang,Mei-Yin Zhang,Shi-Juan Mai,Hui-Yun Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e2204711 PubMed36307901

2022

Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Yun Peng,Dian-Kui Cai,Ren-Li Zeng,Chao-Yang Zhang,Guan-Cheng Li,Si-Fan Chen,Xiao-Qing Yuan,Li Peng

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IDI1 + IDI2 抗体 [EPR17213]

Anti-IDI1 + IDI2 antibody [EPR17213]

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (3)

Abcam product promise