Anti-IDH3G 抗体 (ab118289)
Key features and details
- Goat polyclonal to IDH3G
- Suitable for: WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-IDH3G antibody
IDH3G 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IDH3G -
由来種
Goat -
特異性
ab118289 is expected to recognize both reported isoforms of IDH3G (NP_004126.1; NP_777358.1). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human, Pig
交差が予測される動物種: Cow, Dog
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免疫原
Synthetic peptide:
DHLKLHSYATSIRK
with a Cysteine residue linker, corresponding to internal sequence amino acids 337-350 of Human IDH3G (NP_004126.1; NP_777358.1). -
ポジティブ･コントロール
- MCF7 lysate; Human, Mouse and Rat heart lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab118289 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118289の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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配列類似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 614145 Cow
- Entrez Gene: 481081 Dog
- Entrez Gene: 3421 Human
- Entrez Gene: 25179 Rat
- Omim: 300089 Human
- SwissProt: IDH3G Cow
- SwissProt: P51553 Human
- SwissProt: IDH3G Pig
see all -
別名
- H IDHG antibody
- IDH gamma antibody
- IDH3G antibody
see all
画像
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Western blot - Anti-IDH3G antibody (ab118289)All lanes : Anti-IDH3G antibody (ab118289) at 1 µg/ml
Lane 1 : Rat heart lysate
Lane 2 : Pig heart lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa -
Western blot - Anti-IDH3G antibody (ab118289)Anti-IDH3G antibody (ab118289) at 0.5 µg/ml + MCF7 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab118289 は論文での使用が確認できていません。