AB109588

Anti-IDH2 抗体

Anti-IDH2 antibody

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(1 Publication)

Goat Polyclonal IDH2 antibody. Suitable for WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IDH2 aa 400-450.

別名を表示する

IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2

2 Images

Supplier Data

Western blot - Anti-IDH2 antibody (AB109588)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-IDH2 antibody (ab109588) at 0.1 µg/mL

Lane 1:

Mouse heart tissue lysates at 35 µg

Lane 2:

Rat heart tissue lysates at 35 µg

Lane 3:

Pig heart tissue lysates at 35 µg

Predicted band size: 50 kDa

false

Unknown

Western blot - Anti-IDH2 antibody (AB109588)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-IDH2 antibody (ab109588) at 0.1 µg/mL

All lanes:

Human heart lysate at 35 µg

Predicted band size: 50 kDa

Observed band size: 48 kDa

true

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human, Pig

アプリケーション

applications

免疫原

Synthetic Peptide within Human IDH2 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

P48735

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.

Biological function summary

The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.

Pathways

The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.

The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a role in intermediary metabolism and energy production (PubMed : 19228619, PubMed : 22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed : 19228619, PubMed : 22416140).

See full target information IDH2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicine & pharmacotherapy = Biomedecine & phar 103:9-17 PubMed29635133

2018

Icariside II alleviates oxygen-glucose deprivation and reoxygenation-induced PC12 cell oxidative injury by activating Nrf2/SIRT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Linying Feng,Jianmei Gao,Yuangui Liu,Jingshan Shi,Qihai Gong

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IDH2 抗体

Anti-IDH2 antibody

Western blot - Anti-IDH2 antibody (AB109588)

Western blot - Anti-IDH2 antibody (AB109588)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

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Abcam product promise