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AB95630

Anti-ICOS 抗体 [7E.17G9]

Anti-ICOS antibody [7E.17G9]

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(1 Publication)

Rat Monoclonal ICOS antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Mouse Icos.

別名を表示する

CD278, Ailim, Icos, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD28 and CTLA-4-like protein, CD28-related protein 1, CCLP, CRP-1

1 Images
Flow Cytometry - Anti-ICOS antibody [7E.17G9] (AB95630)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ICOS antibody [7E.17G9] (AB95630)

Staining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b Isotype Control (open histogram) or 0.06 µg of ab95630 (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

7E.17G9

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Nucleic Acid within Mouse Icos. Database link Q9WVS0

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代替製品

Primary Antibodies

AB105227

Anti-ICOS antibody [SP98]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (AB105227)

  • 3
  • 2
Primary Antibodies

AB283488

Alexa Fluor® 555 Anti-ICOS antibody [EPR20560]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-ICOS antibody [EPR20560] (AB283488)

  • 0
  • 0
Primary Antibodies

AB224644

Anti-ICOS antibody [EPR20560]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (AB224644)

  • 1
  • 1
Primary Antibodies

AB237562

Anti-ICOS antibody [EPR22181] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-ICOS antibody [EPR22181] - BSA and Azide free (AB237562)

  • 0
  • 0
Primary Antibodies

AB225577

Anti-ICOS antibody [EPR20560] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] - BSA and Azide free (AB225577)

  • 4
  • 1
Primary Antibodies

AB236875

Anti-ICOS antibody [EPR22181]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-ICOS antibody [EPR22181] (AB236875)

  • 0
  • 0
Primary Antibodies

AB236226

Anti-ICOS antibody [SP98] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] - BSA and Azide free (AB236226)

  • 0
  • 0

組合せ製品

Assay Kits

AB267638

Human ICOS ELISA Kit

Sandwich ELISA - Human ICOS ELISA Kit (AB267638)

  • 0
  • 0
Primary Antibodies

AB216000

Anti-FOXP3 antibody [EPR15038-69] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR15038-69] - BSA and Azide free (AB216000)

  • 5
  • 1
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
Primary Antibodies

AB137132

Anti-PD1 antibody [EPR4877(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD1 antibody [EPR4877(2)] (AB137132)

  • 5
  • 5
Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-ERK1 antibody [Y72] (AB32537)

  • 5
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.06-1 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>A 100 μl volume is recommended for staining the cell sample.</p><p><a href='/products/unavailable/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-bsa-and-azide-free-ab18536'>ab18536</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Inducible T-cell costimulator (ICOS) also known as AILIM or CD278 is a member of the CD28 superfamily of proteins. ICOS is a surface receptor protein approximately 60 kDa in mass. This protein is expressed mainly on activated T cells. It does not appear on naive or resting T cells. ICOS plays an important mechanical role in the immune response as its engagement enhances the production of cytokines and supports cell-cell interactions through its binding to the ICOS ligand.
Biological function summary

ICOS plays a critical role in the regulation of immune responses. It acts as a costimulatory signal for T cells supporting their proliferation and differentiation. ICOS helps form part of a complex involving interactions with the ICOS ligand present on antigen-presenting cells. This interaction affects the function and fate of various T cell subsets including T follicular helper cells which are important for antibody production.

Pathways

ICOS is part of the immune signaling pathway. It associates closely with the CD28 signaling pathway enhancing T-cell activation and survival. ICOS interacts with proteins such as the ICOS ligand (ICOSL) on antigen-presenting cells which helps mediate its effects. These pathways are essential in immune system homeostasis and adaptive immune responses where dysfunction can lead to immune-related diseases.

ICOS has implications in conditions such as autoimmune diseases and cancer. Abnormal expression or function of ICOS relates to the development of autoimmune conditions like systemic lupus erythematosus (SLE) where the overactive immune response damages the body's tissues. Additionally ICOS might support tumor progression in certain types of cancers by promoting immune escape. It interacts with regulatory proteins like CTLA-4 and PD-1 in these diseases which are notably involved in immune checkpoint pathways.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed : 11343123). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed : 10657606). Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed : 36754569). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (PubMed : 27693916). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed : 19915142, PubMed : 27693916). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (PubMed : 11343122).
See full target information Icos
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communications 306:819-25 PubMed12821115

2003

Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Masafumi Nakayama,Norihiro Harada,Ko Okumura,Hideo Yagita
View all publications
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Abcam product promise

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