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AB236975

Anti-ICB1 抗体

Anti-ICB1 antibody

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(2 Publications)

Rabbit Polyclonal ICB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human THEMIS2 aa 50-200.

別名を表示する

C1orf38, ICB1, THEMIS2, Protein THEMIS2, Induced by contact to basement membrane 1 protein, Thymocyte-expressed molecule involved in selection protein 2, Protein ICB-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICB1 antibody (AB236975)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICB1 antibody (AB236975)

Paraffin-embedded human pancreatic cancer tissue stained for ICB1 using ab236975 at 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-ICB1 antibody (AB236975)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ICB1 antibody (AB236975)

NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for ICB1 (green) using ab236975 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human THEMIS2 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q5TEJ8

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組合せ製品

Primary Antibodies

AB33451

Anti-Monocyte + Macrophage antibody [MOMA-2]

  • 4
  • 3
Primary Antibodies

AB126771

Anti-Themis antibody [EPR7353]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Themis antibody [EPR7353] (AB126771)

  • 0
  • 0
Primary Antibodies

AB215729

Anti-GSDMB antibody [EPR20841]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-GSDMB antibody [EPR20841] (AB215729)

  • 0
  • 0
Cell Lines & Lysates

AB266910

Human THEMIS2 (ICB1) knockout A549 cell line

Sanger Sequencing - Human THEMIS2 (ICB1) knockout A549 cell line (AB266910)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
Purity >95%.
バッファー組成
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein ICB1 also known as Intermediate Complex B1 exhibits mechanical functions critical to cellular processes. With a molecular mass of approximately 50 kDa ICB1 is widely expressed in various tissues including the liver brain and muscles. This protein contributes to important mechanistic roles in cells influencing their structural and functional stability. Its distribution across different tissues indicates its involvement in diverse cellular functions.
Biological function summary

The role of ICB1 is linked to cellular signaling pathways and structural maintenance. It often forms part of a larger protein complex within the cytoplasm contributing to cytoskeletal integrity. Through its interactions ICB1 helps in the regulation of intracellular transport and communication. Its integration into multiprotein complexes highlights its importance in maintaining cellular architecture and communication networks.

Pathways

ICB1 participates in signaling cascades such as the MAPK and PI3K-Akt pathways. These pathways are essential for cell survival growth and differentiation. ICB1 interacts with proteins like Akt and ERK1/2 helping to mediate signals that influence cell fate decisions. Its role in these pathways highlights its importance in regulating cell proliferation and responses to external stimuli.

ICB1 has connections to neurodegenerative diseases and certain cancers. In Alzheimer's disease ICB1 is thought to influence the pathology through its interaction with tau proteins contributing to neurofibrillary tangle formation. In some types of cancer alterations in ICB1 expression affect cell cycle regulation promoting unrestrained growth. Understanding the involvement of ICB1 with proteins like tau and cyclin-dependent kinases in these contexts is important for therapeutic development.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production (PubMed : 20644716). Determines the threshold for activation of B cells by low-affinity and low-avidity ligands via PLCG2 activation and its downstream pathways (By similarity).
See full target information THEMIS2
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 29:1834-1849 PubMed35301431

2022

Hypoxia-induced GLT8D1 promotes glioma stem cell maintenance by inhibiting CD133 degradation through N-linked glycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Liu,Liping Jiang,Yulin Shi,Baiyang Liu,Yaomei He,Qiushuo Shen,Xiulin Jiang,Zhi Nie,Jun Pu,Cuiping Yang,Yongbin Chen

Oncogene 41:997-1010 PubMed34974522

2022

Novel function of THEMIS2 in the enhancement of cancer stemness and chemoresistance by releasing PTP1B from MET.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Chieh Huang,Jia-Hau Yen,Yu-Wen Sung,Shiao-Lin Tung,Po-Ming Chen,Pei-Yi Chu,Ya-Chi Shih,Hsiang-Cheng Chi,Yi-Ching Huang,Shih-Jei Huang,Lu-Hai Wang
View all publications
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