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リコンビナントRabMAb

Anti-ICAM5 抗体 [EPR25850-86] (BSA and Azide free) (ab302900)

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Western blot - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
  • Western blot - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
  • Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25850-86] to ICAM5 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free)
    ICAM5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25850-86] to ICAM5 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Tissue lysates: Human, mouse, and rat brain. Whole cell lysates: U-87 MG (human glioblastoma-astrocytoma epithelial cell), HeLa (human cervical adenocarcinoma epithelial cell). IHC-P: Human, mouse, and rat cerebrum, mouse and rat sagittal brain.

  • 特記事項

    ab302900 is a carrier free version of ab302899. 

    ab392899 does not react in: ICC/IF, flow cyt., IP applications with human, mouse, and rat samples. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302900の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 160, 110 kDa (predicted molecular weight: 97 kDa).
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 160, 110 kDa (predicted molecular weight: 97 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 関連性

    ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.

  • 細胞内局在

    Membrane; single-pass type I membrane protein

  • 参照データベース

    • 別名

      • ICAM5 precursor antibody
      • Intercellular adhesion molecule 5 antibody
      • Intercellular adhesion molecule 5 precursor antibody
      • Intercellular adhesion molecule 5, telencephalin antibody
      • Telencephalin antibody
      • TLCN antibody
      • TLN antibody
      see all

    画像

    • Western blot - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      Western blot - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      All lanes : Anti-ICAM5 antibody [EPR25850-86] (ab302899) at 1/1000 dilution

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human liver tissue lysate
      Lane 3 : Human skeletal muscle tissue lysate
      Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
      Lane 5 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 97 kDa
      Observed band size: 160, 110 kDa why is the actual band size different from the predicted?



      This data was developed using ab300422, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The expression profile observed is consistent with what has been described in the literature (PMID: 17682049)
      Low expression: liver, skeletal muscle (PMID: 8995416). 

      Exposure time: Lane 1-3: 92 seconds
      Lane 4-5: 180 seconds

    • Western blot - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      Western blot - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      All lanes : Anti-ICAM5 antibody [EPR25850-86] (ab302899) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Mouse skeletal muscle tissue lysate
      Lane 4 : Rat brain tissue lysate
      Lane 5 : Rat liver tissue lysate
      Lane 6 : Rat skeletal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 97 kDa
      Observed band size: 160, 110 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      This data was developed using ab300422, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Low expression: liver, skeletal muscle (PMID: 8995416)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)

      This data was developed using ab302899, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 dilution (0.505 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human cerebrum was observed. The section was incubated with ab302899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)

      This data was developed using ab302899, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse sagittal brain tissue labeling ICAM5 with ab302899 at 1/1000 dilution (0.505 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse sagittal brain was observed (PMID: 7794412). The section was incubated with ab302899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)

      This data was developed using ab302899, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 dilution (0.505 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebrum was observed. The section was incubated with ab302899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)

      This data was developed using ab302899, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat sagittal brain tissue labeling ICAM5 with ab302899 at 1/1000 dilution (0.505 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat sagittal brain was observed (PMID: 7794412). The section was incubated with ab302899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ olymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)

      This data was developed using ab302899, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 dilution (0.505 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with ab302899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval solution 2) for 20 mins.

    • Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)
      Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (AB302900)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302900 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302900 は論文での使用が確認できていません。

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