Anti-ICAM3 抗体 [ICAM3.1] (ab10804)
Key features and details
- Mouse monoclonal [ICAM3.1] to ICAM3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ICAM3 antibody [ICAM3.1]
ICAM3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ICAM3.1] to ICAM3 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment (Fc-tag) corresponding to ICAM3.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
ICAM3.1 -
ミエローマ
Sp2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10804の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
特記事項 |
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Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 66 kDa.
ICC/IF: Use at an assay dependent dilution From (PubMed:17353362)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. -
配列類似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3385 Human
- Omim: 146631 Human
- SwissProt: P32942 Human
- Unigene: 654563 Human
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別名
- CD50 antibody
- CDW 50 antibody
- CDw50 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (ab10804)Ab10804 staining Human malignant stomach. Staining is localised to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab10804 は 4 報の論文で使用されています。
- Blight BJ et al. Cell Adhesion Molecules are Upregulated and May Drive Inflammation in Chronic Rhinosinusitis with Nasal Polyposis. J Asthma Allergy 14:585-593 (2021). PubMed: 34079296
- Mintz PJ et al. Exploiting human CD34+ stem cell-conditioned medium for tissue repair. Mol Ther 22:149-59 (2014). PubMed: 23985698
- Heasman SJ et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). ICC/IF ; Human . PubMed: 20733052
- Gérard A et al. The Par polarity complex regulates Rap1- and chemokine-induced T cell polarization. J Cell Biol 176:863-75 (2007). PubMed: 17353362