AB206398

Anti-ICAM1 抗体 [EPR19938]

Name: Anti-ICAM1 抗体 [EPR19938] (ab206398) | Abcam
Brand: Abcam Limited
SKU: AB206398
Price: 27000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-ICAM1 antibody [EPR19938]

20ul selling size
RabMAb
Recombinant
詳細を見る

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal ICAM1 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 6 publications.

別名を表示する

CD54, Intercellular adhesion molecule 1, ICAM-1, RICAM-I, Icam1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (AB206398)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (AB206398)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling ICAM1 with ab206398 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on sinusoidal endothelial cells of rat liver [PMID : 17058232].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (AB206398)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ICAM1 with ab206398 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Positive staining on extracellular matrix and blood vessels of rat kidney [PMID : 9198039] [PMID 7902311].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-ICAM1 antibody [EPR19938] (AB206398)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-4 : 5 seconds; Lanes 5/6 : 1 minute.

The observed molecular weight is consistent with the literature (PMID : 1712683).

All lanes:

Western blot - Anti-ICAM1 antibody [EPR19938] (ab206398) at 1/5000 dilution

Lane 1:

Rat spleen lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 4:

L6 (Rat skeletal muscle cell line) whole cell lysate at 10 µg

Lane 5:

Rat liver lysate at 20 µg

Lane 6:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 80-110 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19938

アイソタイプ

IgG

キャリアフリー

交差種

Rat

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM : ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1.

See full target information Icam1

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Pain 164:1965-1975 PubMed37027145

2023

Local analgesia of electroacupuncture is mediated by the recruitment of neutrophils and released β-endorphins.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Tao Shi,Wan-Ying Cao,Xiao-Ning Zhang,Hong-Ye Wan,Yang-Shuai Su,Zheng-Yang Qu,Rui Wang,Wei He,Xiang-Hong Jing,Xiao-Yu Wang

Oxidative medicine and cellular longevity 2022:6934812 PubMed35178159

2022

Serum Pharmacochemistry Combining Network Pharmacology to Discover the Active Constituents and Effect of Xijiao Dihuang Tang Prescription for Treatment of Blood-Heat and Blood-Stasis Syndrome-Related Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Chen,Yang Dai,Jie Xia,Jing Liu,Guisheng Zhou,Cuihua Chen,Baoping Jiang,Lian Yin,Guochun Li

Frontiers in pharmacology 12:561237 PubMed33927611

2021

Danhong Injection Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through the Suppression of the Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Haixia Du,Yu He,Yuanjiang Pan,Mengdi Zhao,Zhiwei Li,Yu Wang,Jiehong Yang,Haitong Wan

Experimental and therapeutic medicine 21:303 PubMed33717246

2021

Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Mei Liu,Jin Zhou,Dan Ji,Jun Yang,Yan-Ping Huang,Qi Wang

Journal of cellular and molecular medicine 23:2849-2862 PubMed30784177

2019

The role of dendritic cells regulated by HMGB1/TLR4 signalling pathway in myocardial ischaemia reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyang Xue,Hanwei Ge,Zhiyong Lin,Hanlei Wang,Wei Lin,Yong Liu,Guowei Wu,Jie Xia,Qifeng Zhao

Scientific reports 8:10346 PubMed29985461

2018

Cobra Venom Factor-induced complement depletion protects against lung ischemia reperfusion injury through alleviating blood-air barrier damage.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Haihua,Wang Wei,Huang Kun,Liao Yuanli,Lin Fei

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ICAM1 抗体 [EPR19938]

Anti-ICAM1 antibody [EPR19938]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (AB206398)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR19938] (AB206398)

Western blot - Anti-ICAM1 antibody [EPR19938] (AB206398)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (6)

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組合せ製品

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Abcam product promise