Anti-ICAM1 抗体 [EPR16608]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (AB179707)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ICAM1 with ab179707 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Positive staining on endothelial cells of mouse kidney is observed [PMID : 17058232].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (AB179707)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ICAM1 with ab179707 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Positive staining on endothelial cells of mouse liver is observed [PMID : 17058232].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-ICAM1 antibody [EPR16608] (AB179707)

Blocking/Dilution buffer : 5% NFDM/TBST.

The observed molecular weight is consistent with the literature (PMID : 1712683).

All lanes:

Western blot - Anti-ICAM1 antibody [EPR16608] (ab179707) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 20 µg

Lane 2:

Mouse liver lysate at 20 µg

Lane 3:

Mouse kidney lysate at 20 µg

Lane 4:

bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate at 10 µg

Lane 5:

A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 80-110 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-ICAM1 antibody [EPR16608] (AB179707)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/2 : 1 second; Lane 3 : 10 seconds.

The observed molecular weight is consistent with the literature (PMID : 1712683).

All lanes:

Western blot - Anti-ICAM1 antibody [EPR16608] (ab179707) at 1/1000 dilution

Lane 1:

Mouse lung lysate at 20 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 3:

WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 80-110 kDa

false

• IHC

CiteAb

Immunohistochemistry - Anti-ICAM1 antibody [EPR16608] (AB179707)

Immunohistochemistry-immunofluorescence using Anti-ICAM1 antibody [EPR16608], ab179707. Publication image from Bertrand, L. et al., 2019, Nat Commun, 31043599. Legend direct from paper.

Disruption of NVU and inflammatory responses in EcoHIV-infected brains. Mice were infected as in Fig. 1, and analyses were performed 3 weeks post infection. Brain sections were stained for ZO-1 (a), laminin (b), ICAM-1 (c), and P-selectin (d). In a, b, n = 6 mice, 20 microvessels per mice, 2 independent experiments. In c, d, n = 6 mice per group, 10 microvessels per mice, 2 independent experiments. e BBB permeability in mock- and EcoHIV-infected mice as assessed by the sodium fluorescein (NaF) extravasation method; n = 10 per group, 2 independent experiments. Whiskers-box plots represent centerline median, with interquartile range and min-max whiskers. Source data are provided as a Source Data file. **p < 0.01 vs mock; unpaired t test. a–d Scale bars : 40 µm

• IHC

CiteAb

Immunohistochemistry - Anti-ICAM1 antibody [EPR16608] (AB179707)

Immunohistochemistry-immunofluorescence using Anti-ICAM1 antibody [EPR16608], ab179707. Publication image from Bertrand, L. et al., 2019, Nat Commun, 31043599. Legend direct from paper.

EcoHIV diminishes post-ischemic stroke NVU recovery. Mice were infected with EcoHIV and subjected to stroke as in Fig. 1. Brain sections were stained for laminin (a), ICAM-1 (b) and P-selectin (c) 24 h post stroke, and quantified for mean fluorescence index (MFI); n = 6 mice per group, 10 microvessels per mice, 2 independent experiments. d Time course of ICAM1 expression levels as quantified by western blotting in sham and ischemic stroke animals at days 1, 4, 7, and 14 post-stroke both in mock (M) and EcoHIV-infected (E) mice. Representative blots are shown, and quantified results from 5–12 samples per group are illustrated on the bar graphs. e Representative image (left) and quantified results (right) of infiltration of the infarct area by Lys6g immunoreactive cells (neutrophils) at 24 h post-ischemic stroke. The sections were also stained for MAP2 (neurons) and Hoechst (nuclei). Absence of MAP2 staining indicates infarct area. Data quantified from 6 mice per group, 2 independent experiments, 4 fields of view per mice at x20 magnification; Z stack images. Whiskers-box plots represent centerline median, with interquartile range and min-max whiskers. Source data are provided as a Source Data file. **p < 0.01; ***p < 0.001; unpaired t test. a–c Scale bars : 40 µm; e scale bar : 320 µm

• WB

CiteAb

Western blot - Anti-ICAM1 antibody [EPR16608] (AB179707)

Western Blotting using Anti-ICAM1 antibody [EPR16608], ab179707. Publication image from Bertrand, L. et al., 2019, Nat Commun, 31043599. Legend direct from paper.

Effective targeting of EcoHIV diminishes post-ischemic stroke inflammation. Mice were infected, treated with ART-7 and ART-11, and subjected to ischemic stroke as in Fig. 6b, c. mRNA levels of cellular activation markers Iba1 (a) and GFAP (b) 7 days post stroke. Protein expression levels of Iba1 (c), GFAP (d), and ICAM1 (e) were quantified by immunoblotting in sham and ischemic stroke animals at day 14 post-stroke in mock (M) and EcoHIV-infected (E) mice that were treated with ART-7 (E + 7) or ART-11 (E + 11). Representative blots are shown, and quantified results are illustrated on the bar graphs. mRNA levels of anti-inflammatory markers ICAM-5 (f) and FoxP3 (g), and tissue degrading enzymes MMP2 (h) and MMP9 (i) were quantified by RT-qPCR. Impact of therapy on viral DNA genome levels was evaluated in ipsilateral hemisphere (j) and spleen (k); n = 5–16 mice per group, 2 independent experiments. Whiskers-box plots represent centerline median, with interquartile range and min-max whiskers. Other graphs represent data as mean and SEM with individual data points. Source data are provided as a Source Data file. *p < 0.05 or **p < 0.01; one-way ANOVA, followed by Tukey multiple comparison test (a, b and f–k), and unpaired t test (c–e)

false

• WB

CiteAb

Western blot - Anti-ICAM1 antibody [EPR16608] (AB179707)

Western Blotting using Anti-ICAM1 antibody [EPR16608], ab179707. Publication image from Bertrand, L. et al., 2019, Nat Commun, 31043599. Legend direct from paper.

EcoHIV diminishes post-ischemic stroke NVU recovery. Mice were infected with EcoHIV and subjected to stroke as in Fig. 1. Brain sections were stained for laminin (a), ICAM-1 (b) and P-selectin (c) 24 h post stroke, and quantified for mean fluorescence index (MFI); n = 6 mice per group, 10 microvessels per mice, 2 independent experiments. d Time course of ICAM1 expression levels as quantified by western blotting in sham and ischemic stroke animals at days 1, 4, 7, and 14 post-stroke both in mock (M) and EcoHIV-infected (E) mice. Representative blots are shown, and quantified results from 5–12 samples per group are illustrated on the bar graphs. e Representative image (left) and quantified results (right) of infiltration of the infarct area by Lys6g immunoreactive cells (neutrophils) at 24 h post-ischemic stroke. The sections were also stained for MAP2 (neurons) and Hoechst (nuclei). Absence of MAP2 staining indicates infarct area. Data quantified from 6 mice per group, 2 independent experiments, 4 fields of view per mice at x20 magnification; Z stack images. Whiskers-box plots represent centerline median, with interquartile range and min-max whiskers. Source data are provided as a Source Data file. **p < 0.01; ***p < 0.001; unpaired t test. a–c Scale bars : 40 µm; e scale bar : 320 µm

false