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リコンビナントRabMAb

Anti-ICAM1 抗体 [EP1442Y] (ab53013)

Submit a review Q&A (5)References (59)
Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
  • Anti-ICAM1 antibody [EP1442Y] (ab53013)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1442Y] to ICAM1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

製品の概要

  • 製品名

    Anti-ICAM1 antibody [EP1442Y]
    ICAM1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1442Y] to ICAM1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human ICAM1 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab218845)

  • ポジティブ･コントロール

    • WB: Huvec, Ramos and Raji whole cell lysate; IHC: Human kidney and tonsil tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab53013の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

  • 配列類似性

    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

  • 翻訳後修飾

    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54) antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    画像

    • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      All lanes : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : ICAM1 CRISPR-Cas9 edited HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Ramos cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 58 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-ICAM1 antibody [EP1442Y] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab53013 was shown to bind specifically to ICAM1. A band was observed at 90 kDa in wild-type HeLa cell lysates with no signal observed at this size in Icam1 CRISPR-Cas9 edited cell line ab261742 (CRISPR-Cas9 edited cell lysate ab256947). The band observed in the CRISPR-Cas9 edited lysate lane below 90 kDa (not observed by this antibody) is likely to represent a truncated form of ICAM1. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and Icam1 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling ICAM1 with purified ab53013 at 1/500 dilution (0.22 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling ICAM1 with purified ab53013 at 1/500 dilution (0.22 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Lanes 1 & 3 : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution
      Lane 2 : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution (Purified)

      Lane 1 : Untreated HUVEC (Human umbilical vein endothelial cell) whole cell lysate
      Lane 2 : HUVEC (Human umbilical vein endothelial cell) treated with 50ng/ml TNF-a for 24 hours whole cell lysate
      Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 58 kDa



      The molecular mass observed is consistent with what has been described in the literatures (PMID: 29777158, 30082828, 31244919).

    • Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Western blot - Anti-ICAM1 antibody [EP1442Y] (ab53013)
      All lanes : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : ICAM1 knockout HeLa cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Ramos cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 58 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab53013 observed at 90 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab53013 Anti-ICAM1 antibody [EP1442Y]  was shown to specifically react with ICAM1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261742 (knockout cell lysate ab256947) was used. Wild-type and ICAM1 knockout samples were subjected to SDS-PAGE. ab53013 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Anti-ICAM1 antibody [EP1442Y] (ab53013)
      Anti-ICAM1 antibody [EP1442Y] (ab53013)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (59)

    ab53013 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab53013 は 59 報の論文で使用されています。

    • Guo Z  et al. SIRT6 deficiency in endothelial cells exacerbates oxidative stress by enhancing HIF1α accumulation and H3K9 acetylation at the Ero1α promoter. Clin Transl Med 13:e1377 (2023). PubMed: 37598403
    • Morimoto M  et al. miR-1246 in tumor extracellular vesicles promotes metastasis via increased tumor cell adhesion and endothelial cell barrier destruction. Front Oncol 13:973871 (2023). PubMed: 37124539
    • Williams D  et al. Stable flow-induced expression of KLK10 inhibits endothelial inflammation and atherosclerosis. Elife 11:N/A (2022). PubMed: 35014606
    • Jhang JF  et al. Improved Urothelial Cell Proliferation, Cytoskeleton and Barrier Function Protein Expression in the Patients With Interstitial Cystitis/Bladder Pain Syndrome After Intravesical Platelet-Rich Plasma Injection. Int Neurourol J 26:S57-67 (2022). PubMed: 35073671
    • Yang Y  et al. A functional SNP rs895819 on pre-miR-27a is associated with bipolar disorder by targeting NCAM1. Commun Biol 5:309 (2022). PubMed: 35379867
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Question

    Product code: 52895
    Lot number: GR27346-6
    Inquiry: I have noticed that you provide a concentration for some of your antibodies whilst others simply have a volume? I am using the following antibodies.. CD11a - ab52895, Lot GR27346-6 CD54 - ab53013 Lot GR10519-3 Which just have a volume, Do you have access to concentration information for these antibodies? Also is this likely to change if I receive vials from a different 'lot' in the future? Thanks,

    Read More
    Answer

    Thank you for contacting us.

    The concentration for lot GR27346-6 is XXXX mg/ml.

    The conc. for lot GR10519-3 is XXXX mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    No signal in IHC on human tissue

    Read More
    Answer

    Thank you for calling Abcam earlier today.

    I am sorry that you were having issue with ab53013 in IHC. As we discussed on the telephone, I am sending you a vial of ab109361 as a free of charge replacement. Your new order number for tracking purposes only is *.

    If there is anything else I can help you with, or if you experience any issues with the replacement antibody, then please let me know.

    Read More

    Question

    Thank you for your below email and for the offer of a free replacement. Our customer has accepted your offer.

    Please include the free replacement in one of our next shipments.

    Thank you in advance.

    Kind regards,

    Read More
    Answer

    Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

    Weare pleased to arrange a replacement product free of charge. I have added now one free unit of ab53013,xx We are currently awaiting the vial to be transferred from the US office.To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should youthe customer stillbe experiencing difficulties, or if you have any further questions, please do not hesitate to let us know

    Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

    Read More

    Question

    Dear sir/madam,

    In accordance with your request, we have filled in the technical questionnaire. We hope this will give you all the information needed to understand the problem.

    We look forward to hearing your response.

    Best regards,

    Read More
    Answer

    Thank you very muchfor taking time to complete our questionnaire and the additional information. Even though we have not yet tested this antibody in frozen sections, I would expect the new batch to work as the previous one as this is a monoclonal antibody. Indeed it appears that you may have received a faulty vial. I am very sorry for this inconvenience. As said before, we do not have received any negative feedback on this antibody during the last three years. The vial you received might has been damaged during the transport or storage.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation. We do have currently the lot GR10519-7 in stock in our US office. Would you like to receive a new vial of this lot?

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    One of our customers is using the anti ICAM-1 antibody Ab53013. Previously they had a good staining on both IHC-P/F of human skin biopsies with Lot.# GR10519-2

    With the use of the new lot.# GR10519-6 they did not get any staining, while the secondary antibody worked well on other antibodies in the same setting.

    Please advise on how to handle this problem. Is there any problem described for this lot.#?

    Thank you in advance.



    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We have not received any product complaints of this antibody during the past three years and have also no other indication that there might a problem with a batch. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.


    Even though in this case it seems to be a straigthforward batch issue, I would nevertheless appreciate if we could gather more details of the experimental procedure. It should not take more than 5 to 10 minutes to the customer. I am attaching therefore our questionnaire which will help to put together somefurther information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results - which I do not expect in this case. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


    I look forward to receiving your reply.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com