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AB289370

Anti-Iba1 抗体 [HL22] - BSA and Azide free

Anti-Iba1 antibody [HL22] - BSA and Azide free

3

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal Iba1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AIF1.

別名を表示する

G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, iba1, iba-1, iba 1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunofluorescence analysis of THP-1 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Iba1 protein at cytoplasm using ab289370 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for Iba1 using ab289370 diluted at 1 : 100 (brown).

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labelling Iba1 protein with ab289370 at a 1/200 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue stained for Iba1 using ab289370 diluted at 1 : 1000 (brown).

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of frozen mouse brain tissue stained for Iba1 using ab289370 diluted at 1 : 100 (green). Iba1 antibody [HL22-RT] diluted at 1 : 100 (red).

Nuclear stain is DAPI (blue).

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of frozen mouse spinal cord tissue stained for Iba1 using ab289370 diluted at 1 : 200 (green).

Nuclear stain is DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [HL22] - BSA and Azide free (AB289370)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue stained for Iba1 using ab289370 diluted at 1 : 1000 (brown).

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

HL22

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, ICC/IF, IHC-Fr, WB

applications

免疫原

Synthetic Peptide within Human AIF1. The exact immunogen used to generate this antibody is proprietary information.

P55008

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組合せ製品

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

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Primary Antibodies

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Anti-NeuN antibody [EPR12763] - Neuronal Marker

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Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

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Proteins & Peptides

AB114149

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Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

  • 0
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Primary Antibodies

AB209064

Anti-TMEM119 antibody [28-3] - Microglial marker

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Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (AB209064)

  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Iba1 also known as Allograft Inflammatory Factor 1 (AIF-1) is a 17 kDa protein notable for its involvement in immune responses. It is expressed mainly in microglia and macrophages key components of the central nervous system's immune system. Researchers often use Iba1 in Western blots and immunohistochemistry (IHC) to study microglial activation. The protein's expression is a strong marker of microglial activation making it a focal point in neuroinflammation studies. Anti-Iba1 antibodies have gained popularity for their effectiveness in identifying microglia in brain tissue sections through Iba1 staining techniques.
Biological function summary

Iba1 modulates actin bundling facilitating changes in microglial morphology and motility. It does not form part of a multiprotein complex but is significantly involved in cytoskeletal dynamics. This protein's expression increases during brain injury indicating its role in response to neural trauma. Iba1 has calcium-binding properties which suggest that it might interact with proteins involved in calcium signaling pathways.

Pathways

Calcium signaling and actin cytoskeletal rearrangement are significant biological processes that involve Iba1. Within these pathways Iba1 interacts closely with other actin-binding proteins that affect microglial movement and morphology. Such interactions illustrate how Iba1 helps microglia navigate and respond to changes within the brain environment.

Iba1 is often associated with neurodegenerative diseases like Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these conditions elevated Iba1 levels mark increased microglial activity which correlates with disease progression. Connections with proteins such as amyloid-beta in Alzheimer's disease suggest its role in mediating inflammatory responses and linking inflammation to disease pathology.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
See full target information AIF1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 20:2611-2623 PubMed39314167

2024

High-dose dexamethasone regulates microglial polarization via the GR/JAK1/STAT3 signaling pathway after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mengshi Yang,Miao Bai,Yuan Zhuang,Shenghua Lu,Qianqian Ge,Hao Li,Yu Deng,Hongbin Wu,Xiaojian Xu,Fei Niu,Xinlong Dong,Bin Zhang,Baiyun Liu

Nature communications 15:7855 PubMed39245680

2024

Human neural stem cell-derived artificial organelles to improve oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Wang,Mengke Zhao,Meina Wang,Dong Fu,Lin Kang,Yu Xu,Liming Shen,Shilin Jin,Liang Wang,Jing Liu
View all publications
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

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