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リコンビナント

Anti-Iba1 抗体 [EPR16588] - Goat IgG (Chimeric) (ab289874)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
  • Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Goat Chimera monoclonal [EPR16588] to Iba1 - Chimeric
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free Unconjugated

製品の概要

  • 製品名

    Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric)
    Iba1 一次抗体 製品一覧

  • 製品の詳細

    Goat Chimera monoclonal [EPR16588] to Iba1 - Chimeric

  • 由来種

    Goat Chimera

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human tonsil, Human liver, Human cerebrum, Mouse liver, Mouse cerebrum, Rat liver and Rat cerebrum tissues. ICC/IF: THP-1 cells. Flow Cyt (Intra): THP-1 cells

  • 特記事項

    This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab178846). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289874の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/1000.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
特記事項
Flow Cyt (Intra)
1/1000.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

ターゲット情報

  • 機能

    Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

  • 組織特異性

    Detected in T-lymphocytes and peripheral blood mononuclear cells.

  • 配列類似性

    Contains 2 EF-hand domains.

  • 翻訳後修飾

    Phosphorylated on serine residues.

  • 細胞内局在

    Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AIF 1 antibody
    • AIF-1 antibody
    • Aif1 antibody
    • AIF1 protein antibody
    • AIF1_HUMAN antibody
    • Allograft inflammatory factor 1 antibody
    • Allograft inflammatory factor 1 splice variant G antibody
    • allograft inflammatory factor-1 splice variant Hara-1 antibody
    • balloon angioplasty responsive transcription antibody
    • BART 1 antibody
    • G1 antibody
    • G1 putative splice variant of allograft inflamatory factor 1 antibody
    • IBA 1 antibody
    • IBA1 antibody
    • interferon gamma responsive transcript antibody
    • Interferon responsive transcript 1 antibody
    • interferon responsive transcript factor 1 antibody
    • Ionized calcium binding adapter molecule 1 antibody
    • Ionized calcium-binding adapter molecule 1 antibody
    • ionized calcium-binding adapter molecule antibody
    • IRT 1 antibody
    • IRT1 antibody
    • Microglia response factor antibody
    • MRF1 antibody
    • Protein g1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Iba1 with ab289874 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on human tonsil. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 minutes. Immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Iba1 with ab289874 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on Kupffer cells in the human liver. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 minutes. Immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Iba1 with ab289874 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on microglia in the human cerebrum. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 minutes. Immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Iba1 with ab289874 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on Kupffer cells in the mouse liver. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 minutes. Immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Iba1 with ab289874 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on microglia in the mouse cerebrum. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 minutes. Immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Iba1 with ab289874 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on Kupffer cells in the rat liver. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 minutes immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Iba1 with ab289874 at 1/1000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on microglia in the rat cerebrum. The section was incubated with ab289874 for 30 mins at room temperature and followed by specific anti-goat IgG antibody (ab97101) for 8 minutes. Immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Immunocytochemistry/ Immunofluorescence - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells lebelling Iba1 with ab289874 at 1/100 dilution (9.38 µg/ml), followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 dilution (Green). Confocal image showing cytoplasmic and membranous staining in THP-1 cell line. Negative control: MCF7 (PMID: 30386176). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: PBS was used instead of primary antibody followed by secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 dilution.

  • Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Flow Cytometry (Intracellular) - Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling Iba1 with ab289874 at 1/1000 dilution (Red) compared with a Goat IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Donkey anti-Goat IgG (Alexa Fluor® 488, ab150133) at 1/2000 dilution was used as the secondary antibody. Negative control: MCF7 (PMID: 30386176).

  • Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)
    Anti-Iba1 antibody [EPR16588] - Goat IgG (Chimeric) (ab289874)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab289874 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab289874 は 1 報の論文で使用されています。

  • Jia M  et al. Liraglutide ameliorates delirium-like behaviors of aged mice undergoing cardiac surgery by mitigating microglia activation via promoting mitophagy. Psychopharmacology (Berl) 241:687-698 (2024). PubMed: 37968531

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