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Anti-IB-1 抗体 (ab24449)

Submit a review Q&A (6)References (6)
Western blot - Anti-IB-1 antibody (ab24449)

    Key features and details

    • Rabbit polyclonal to IB-1
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-IB-1 antibody
      IB-1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to IB-1

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse, Rat, Human

    • 免疫原

      Synthetic peptide corresponding to Mouse IB-1 (N terminal).

    • ポジティブ･コントロール

      • Mouse Intestine. Rat kidney. Jurkat whole cell lysate (ab7899).

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called 3664 JIP-1 Antibody.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      Preservative: 0.02% Thimerosal (merthiolate)
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
    • Concentration information loading...

    • 精製度

      Protein A purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab24449の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). A band of 110 kDa can also be observed in human samples.
    特記事項
    WB
    Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). A band of 110 kDa can also be observed in human samples.

    ターゲット情報

    • 関連性

      Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. Tissue specificity: Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis. Disease: Diabetes mellitus, non-insulin-dependent Similarity: Belongs to the JIP scaffold family. Contains 1 PID domain. Contains 1 SH3 domain. Domain: The destruction boxes (D-box) may act as recognition signals for degradation via the ubiquitin-proteasome pathway. A minimal inhibitory domain prevents pancreatic beta cell apoptosis in vitro, and prevents activation of c-jun by MAPK8, MAPK9 and MAPK10. The SH3 domain mediates homodimerization. PTM: Phosphorylated by MAPK8, MAPK9 and MAPK10. Phosphorylation on Thr-103 is also necessary for the dissociation and activation of MAP3K12. Phosphorylated by isoform 1 and isoform 2 of VRK2. Hyperphosphorylated during mitosis following activation of stress-activated and MAP kinases. Ubiquitinated. Two preliminary events are required to prime for ubiquitination; phosphorylation and an increased in intracellular calcium concentration. Then, the calcium influx initiates ubiquitination and degradation by the ubiquitin-proteasome pathway.

    • 細胞内局在

      Cytoplasmic. Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus

    • 参照データベース

      see all

    • 別名

      • C jun amino terminal kinase interacting protein 1 antibody
      • C-jun-amino-terminal kinase-interacting protein 1 antibody
      • IB 1 antibody
      • IB-1 antibody
      • IB1 antibody
      • Islet brain 1 antibody
      • Islet-brain 1 antibody
      • JIP 1 antibody
      • JIP-1 antibody
      • JIP1_HUMAN antibody
      • JNK interacting protein 1 antibody
      • JNK MAP kinase scaffold protein 1 antibody
      • JNK-interacting protein 1 antibody
      • MAPK8IP 1 antibody
      • MAPK8IP1 antibody
      • MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1 antibody
      • Mitogen activated protein kinase 8 interacting protein 1 antibody
      • Mitogen-activated protein kinase 8-interacting protein 1 antibody
      • PRKM8 antibody
      • PRKM8 interacting protein antibody
      • PRKM8IP antibody
      see all

    画像

    • Western blot - Anti-IB-1 antibody (ab24449)
      Western blot - Anti-IB-1 antibody (ab24449)
      All lanes : Anti-IB-1 antibody (ab24449)

      Lane 1 : Rat kidney
      Lane 2 : Mouse Intestine
      Lane 3 : Jurkat Cell lysate

      Predicted band size: 78 kDa
      Observed band size: 78 kDa
      Additional bands at: 110 kDa (possible isoform)

    参考文献 (6)

    ab24449 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab24449 は 6 報の論文で使用されています。

    • Saeed R  et al. Expression Silencing of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 Conferred Its Role in Pancreatic β-Cell Physiology and Insulin Secretion. Metabolites 13:N/A (2023). PubMed: 36837926
    • Saeed R  et al. Dual Role of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 in Inflammasome and Pancreatic β-Cell Function. Int J Mol Sci 24:N/A (2023). PubMed: 36902422
    • Musi CA  et al. Colocalization and Interaction Study of Neuronal JNK3, JIP1, and β-Arrestin2 Together with PSD95. Int J Mol Sci 23:N/A (2022). PubMed: 35456931
    • Goodwani S  et al. Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions. Int J Mol Sci 21:N/A (2020). WB ; Mouse . PubMed: 32659913
    • Zhao L  et al. E6-induced selective translation of WNT4 and JIP2 promotes the progression of cervical cancer via a noncanonical WNT signaling pathway. Signal Transduct Target Ther 4:32 (2019). PubMed: 31637011
    • Sherman MA  et al. Soluble Conformers of Aß and Tau Alter Selective Proteins Governing Axonal Transport. J Neurosci 36:9647-58 (2016). PubMed: 27629715

    レビューと Q&A

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    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    The invoice for this antibody () has already been paid.

    We would like to held this credit on the account for use on a future order.

    Please email the credit note directly to our group leader professor
    Thanks

    Read More
    Answer

    Thank you for contacting us again.
    Please contact my colleges from the financial department, as I can raise a credit note, but everything else is in their hands.

    It may be useful to contact the Apotheke as well to inform that this credit note is exclusively for your group and not to be used for paying outstanding invoices (we know from experience that many university purchasing agent do this).
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

    Read More

    Question

    After discussing this with our boss, we have decided we would like to have a refund for this antibody.

    Could you please inform me about the procedure in order to do so?

    Thanks

    Read More
    Answer

    Thank you for contacting us.
    Your credit note ID is 22405.
    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2) Held on the account for use against a future order.
    (3) A full refund can be offered where no other invoices are outstanding.
    Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
    To specifically receive a refund please contact Tel# 030 896 779 154.
    The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Question

    Dear Anja

    Attached you will find the information you required. The images of GFP-JIP1 transfected neurons stained with anti-JIP1 show almost no co-localization.

    1) Abcam product code ab 24449

    2) Abcam order reference number or product batch number
    Purchase order number: 30072012
    3) Description of the problem the staining is unspecific, looks like tubulin, but is in rat hippocampal neurons not localized to the axon tip (like expected and observed with JIP1-GFP. Shows also no signif. Co-localization with JIP1-GFP!
    4) Sample preparation: primary neurons prepared to common protocols and seeded on poly-ornithin coated coverslips like usual. cells fixed with 4% PFA-succrose, blocked with NGS
    Species rat,
    Type of sample: Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed paraffin embedded sections, cells in culture, other: primary hippocampal neurons, fixed with PFA(4%) -Succrose
    Sample preparation standard protocol for primary neuron culture
    Positive control JIP1-GFP transfected into the neuron by AMAXA electroporation
    Negative control secondary ab alone (alexa 488/594, Invitrogen)
    5) Fixation step
    Yes/No
    If yes: Fixative agent and concentration PFA 4% - succrose
    Fixation time 18 min
    Fixation temperature RT
    6) Antigen retrieval method no, not recommended for this cells
    7) Permeabilization method:
    Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers? Yes
    Permeabilizing agent and concentration:0.1% Triton-X, 0.1% Na-Citrate in PBS for 4 min at RT

    8) Blocking agent (eg BSA, serum…): 10% NGS in PBS
    Concentration
    Blocking time 1h
    Blocking temperature RT
    9) Endogenous peroxidases blocked? no
    Endogenous biotins blocked? no
    10) Primary antibody (If more than one was used, describe in “additional notes”) :
    Concentration or dilution 1:40 (tried also 1:100; 1:25)
    Diluent buffer 5% NGS in PBS
    Incubation time ON at 4°C
    11) Secondary antibody:
    Species: goat
    Reacts against: rabbit
    Concentration or dilution 1:1000
    Diluent buffer PBS
    Incubation time 90 min
    Fluorochrome or enzyme conjugate alexa 488/ alexa 594
    12) Washing after primary and secondary antibodies:
    Buffer pbs
    Number of washes 4
    13) Detection method fluorescence
    14) How many times have you run this staining? 2x
    Do you obtain the same results every time? yes
    What steps have you altered to try and optimize the use of this antibody? Changed concentrations; added tween-20; included JIP1-GFP transfection as positive control
    Document attachment: Attaching images of your IHC is strongly recommended and can greatly speed up our investigation of your problem. Please find attached a pic with neurons transfected with JIP1-GFP (green), staind with JIP1 ab (ab24449) +Alexa 594 (red)

    Read More
    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
    As Anja is in her holidays, i will deal with your case.
    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.
    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial. In addition, Anja had reviewed the images from the lab on rat neuron cells and the results fit your discribtion of the localization.
    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.
    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    Phoned because ab24449 gave wrong band sizes in mouse tissues in WB.

    Read More
    Answer

    Thank you for contacting Abcam.

    I am sorry that this product did not perform correctly for you. As we had spoken about, if this product has been purchased within the last six months it will be covered by our Abpromise guarantee and I would be able to offer you a free of charge replacement for this. When you have decided which product you would like for that replacement please let me know either by phone or by email. In order to process the replacement we will need the Abcam order confirmation number as well.

    Please let me know if you have any questions or concerns. I look forward to your reply.

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    Question

    Hi there, I am considering purchasing this antibody and was wondering if this antibody can be used for immunoprecipitation experiments. Thanks, jane

    Read More
    Answer

    Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in immunoprecipitation. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    I am interested in testing whether your anti-JIP1 polyclonal antibody could work for detecting the homologue in Drosophila. Your product info says the antigen was a small peptide. Could you tell me the sequence of the peptide (or what part of mouse JIP1 it represented)? If it was raised against a sequence that is conserved between mouse and drosophila we would purchase it and test it. Thank you very much!

    Read More
    Answer

    I'm afraid I have been quite unsuccessful at finding out the information you needed. The source of the antibody did not wish to divulge to me the exact sequence and I was only able to find out that it was the N terminal part of the protein which was used. I'm sorry I couldn't help you more on this occasion,

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