Anti-iASPP 抗体 (ab34898)
Key features and details
- Rabbit polyclonal to iASPP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-iASPP antibody
iASPP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to iASPP -
由来種
Rabbit -
特異性
A BLAST analysis was used to suggest cross reactivity with iASPP proteins from human, dog and mouse sources. Expect reactivity against both isoform 1 and isoform 2. Partial reactivity may occur against iASPP from bovine. Reactivity against homologues from other sources is not known.
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アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow
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免疫原
Synthetic peptide:
R EGESVTVLRR DGPEETD
, corresponding to amino acids 780-797 of Human iASPP (Isoform 1). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity-purified antibody is directed against the human iASPP protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34898の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (5) |
1/1000 - 1/5000. Detects a band of approximately 102 kDa (predicted molecular weight: 90 kDa).
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| 特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/5000. Detects a band of approximately 102 kDa (predicted molecular weight: 90 kDa). |
ターゲット情報
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関連性
ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 505818 Cow
- Entrez Gene: 10848 Human
- Entrez Gene: 333654 Mouse
- Omim: 607463 Human
- SwissProt: Q8WUF5 Human
- SwissProt: Q5I1X5 Mouse
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別名
- IASPP antibody
- inhibitor of apoptosis stimulating protein of p53 antibody
- Inhibitor of ASPP protein antibody
see all
画像
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Western blot - Anti-iASPP antibody (ab34898)Anti-iASPP antibody (ab34898) at 1/1500 dilution (at 4°C, o/n) + 35ug of MCF7 whole cell lysate
Secondary
IRDye™800 conjugated anti-Rabbit IgG diluted 1:10000, for 45 min at RT
Predicted band size: 90 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iASPP antibody (ab34898)IHC image of ab34898 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34898, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab34898 は 17 報の論文で使用されています。
- Tulbah S et al. Variable phenotype of a null PPP1R13L allele in children with dilated cardiomyopathy. Am J Med Genet A 194:59-63 (2024). PubMed: 37698259
- Hai B et al. LncRNA XIST Promotes Growth of Human Chordoma Cells by Regulating miR-124-3p/iASPP Pathway. Onco Targets Ther 13:4755-4765 (2020). PubMed: 32547104
- Webster MR et al. Paradoxical Role for Wild-Type p53 in Driving Therapy Resistance in Melanoma. Mol Cell 77:633-644.e5 (2020). PubMed: 31836388
- Liu ZZ et al. Downregulation of iASPP Expression Suppresses Proliferation, Invasion and Increases Chemosensitivity to Paclitaxel of Head and Neck Squamous Cell Carcinoma In Vitro. Chin Med Sci J 34:184-193 (2019). PubMed: 31601301
- Zou H et al. miR-129 targets CDK1 and iASPP to modulate Burkitt lymphoma cell proliferation in a TAp63-dependent manner. J Cell Biochem 119:9217-9228 (2018). PubMed: 30105797