Anti-Hydrogen Potassium ATPase Beta/ATP4B 抗体 [EPR8819(2)] - C-terminal (ab186749)
![Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)](https://www.abcam.co.jp/ps/products/186/ab186749/Images/ab186749-219971-Picture19.jpg "Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8819(2)] to Hydrogen Potassium ATPase Beta/ATP4B - C-terminal
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal
Hydrogen Potassium ATPase Beta/ATP4B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8819(2)] to Hydrogen Potassium ATPase Beta/ATP4B - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human Hydrogen Potassium ATPase Beta/ATP4B recombinant protein; Human stomach and HepG2 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8819(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186749の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 33 kDa).
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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機能
Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity. -
配列類似性
Belongs to the X(+)/potassium ATPases subunit beta family. -
ドメイン
The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 496 Human
- Omim: 137217 Human
- SwissProt: P51164 Human
- Unigene: 434202 Human
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別名
- Gastric H(+)/K(+) ATPase subunit beta antibody
- ATP4B antibody
- ATP4B_HUMAN antibody
see all
画像
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Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749) at 1/1000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDaBlocking buffer: 5% NFDM/TBST
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![Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)](https://www.abcam.co.jp/ps/products/186/ab186749/Images/ab186749-219970-Picture18.jpg)
Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749) at 1/5000 dilution + Human stomach lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 33 kDaBlocking buffer: 5% NFDM/TBST
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![Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)](https://www.abcam.co.jp/ps/products/186/ab186749/Images/ab186749-219969-Picture17.jpg)
Western blot - Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749) at 1/1000 dilution + Human Hydrogen Potassium ATPase Beta/ATP4B recombinant full length protein with proprietary tag at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDaBlocking buffer: 5% NFDM/TBST
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![Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)](https://www.abcam.co.jp/ps/products/186/ab186749/Images/ab186749-4-benefits-of-recombinant-antibodies.png)
Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab186749 は論文での使用が確認できていません。