Anti-HUWE1/Mule 抗体 (ab70161)
Key features and details
- Rabbit polyclonal to HUWE1/Mule
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HUWE1/Mule antibody
HUWE1/Mule 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HUWE1/Mule -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human HUWE1/Mule aa 2250-2300.
Database link: Q7Z6Z7 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70161の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Detects a band of approximately >460 kDa (predicted molecular weight: 481 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately >460 kDa (predicted molecular weight: 481 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. -
組織特異性
Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Defects in HUWE1 are the cause of mental retardation syndromic X-linked Turner type (MRXST) [MIM:300706]; also known as mental retardation and macrocephaly syndrome. MRXST shows clinical variability. Associated phenotypes include macrocephaly and variable contractures.
A chromosomal microduplication involving HUWE1 and HSD17B10 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation. -
配列類似性
Belongs to the TOM1/PTR1 family.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 UBA domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 WWE domain. -
ドメイン
The HECT domain mediates inhibition of the transcriptional activity of p53. -
翻訳後修飾
Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells. - Information by UniProt
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参照データベース
- Entrez Gene: 465650 Chimpanzee
- Entrez Gene: 10075 Human
- Entrez Gene: 59026 Mouse
- SwissProt: Q7Z6Z7 Human
- SwissProt: Q5BMM7 Mouse
- SwissProt: Q7TMY8 Mouse
- Unigene: 136905 Human
- Unigene: 27372 Mouse
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別名
- ARF binding protein 1 antibody
- ARF BP1 antibody
- ARF-binding protein 1 antibody
see all
画像
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Western blot - Anti-HUWE1/Mule antibody (ab70161)All lanes : Anti-HUWE1/Mule antibody (ab70161) at 1/10000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 481 kDaDetection: Chemiluminescence with an exposure time of 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUWE1/Mule antibody (ab70161)Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded section of human ovarian carcinoma tissue labelling HUWE1/Mule with ab70161 at 1/5000 (0.2µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-HUWE1/Mule antibody (ab70161)HUWE1/Mule was immunoprecipitated from HeLa whole cell lysate with 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab70161 at 1 µg/ml dilution.
Lane 1: ab70161 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HUWE1/Mule antibody (ab70161)IHC image of ab70161 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70161, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab70161 は 17 報の論文で使用されています。
- Amici DR et al. The HAPSTR2 retrogene buffers stress signaling and resilience in mammals. Nat Commun 14:152 (2023). PubMed: 36631436
- Yuan Y et al. The E3 ubiquitin ligase HUWE1 acts through the N-Myc-DLL1-NOTCH1 signaling axis to suppress glioblastoma progression. Cancer Commun (Lond) 42:868-886 (2022). PubMed: 35848447
- Molkentine DP et al. p16 Represses DNA Damage Repair via a Novel Ubiquitin-Dependent Signaling Cascade. Cancer Res 82:916-928 (2022). PubMed: 34965932
- Yi J et al. Parthenolide and arsenic trioxide co-trigger autophagy-accompanied apoptosis in hepatocellular carcinoma cells. Front Oncol 12:988528 (2022). PubMed: 36353537
- Endres T et al. Ubiquitylation of MYC couples transcription elongation with double-strand break repair at active promoters. Mol Cell 81:830-844.e13 (2021). PubMed: 33453168