Anti-HUS1 抗体 (ab186850)
Key features and details
- Rabbit polyclonal to HUS1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HUS1 antibody
HUS1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HUS1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human HUS1.
Database link: O60921 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186850の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| WB |
1/500 - 1/2000. Predicted molecular weight: 32 kDa.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 32 kDa. |
ターゲット情報
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機能
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the HUS1 family. -
細胞内局在
Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3364 Human
- Omim: 603760 Human
- SwissProt: O60921 Human
- Unigene: 152983 Human
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別名
- Checkpoint protein HUS1 antibody
- hHUS1 antibody
- HUS1 (S. pombe) checkpoint homolog antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (ab186850)
Immunohistochemistry of paraffin-embedded human stomach cancer using ab186850 at dilution of 1:200 (x400 lens).
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Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (ab186850)Immunohistochemistry of paraffin-embedded human kidney using ab186850 at dilution of 1:200 (x400 lens).
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Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (ab186850)Immunofluorescence analysis of U2OS cell using ab186850. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
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Immunocytochemistry/ Immunofluorescence - Anti-HUS1 antibody (ab186850)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186850. Blue DAPI for nuclear staining. -
Western blot - Anti-HUS1 antibody (ab186850)All lanes : Anti-HUS1 antibody (ab186850) at 1/500 dilution
Lane 1 : OVCAR3 cell extract
Lane 2 : K562 cell extract
Lane 3 : 293T cell extract
Predicted band size: 32 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab186850 は論文での使用が確認できていません。