Anti-HURP 抗体 (ab84509)
Key features and details
- Rabbit polyclonal to HURP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HURP antibody
HURP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HURP -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human HURP aa 200-300.
Database link: Q15398 -
ポジティブ･コントロール
- IHC-P: Human basal cell carcinoma and human seminoma tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84509の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500.
|
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| WB |
1/200.
|
| 特記事項 |
|---|
|
IHC-P
1/100 - 1/500. |
|
WB
1/200. |
ターゲット情報
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機能
Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. -
組織特異性
Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta. -
配列類似性
Belongs to the SAPAP family. -
発生段階
Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells. -
翻訳後修飾
Ubiquitinated, leading to its degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells. - Information by UniProt
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参照データベース
- Entrez Gene: 9787 Human
- SwissProt: Q15398 Human
- Unigene: 77695 Human
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別名
- DAP-5 antibody
- DAP5 antibody
- Discs large homolog 7 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HURP antibody (ab84509)ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HURP antibody (ab84509)ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry, using DAB staining. -
Western blot - Anti-HURP antibody (ab84509)Anti-HURP antibody (ab84509) at 1/200 dilution + 25µg of WCE from U20S cells synchronized by thimidine arrest and release to enrich in mitotic cells (same on lane 1 and 2).
Image kindly provided by Patrizia Lavia, CNR National Research Council, University "La Sapienza", Rome, Italy.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab84509 は 3 報の論文で使用されています。
- Zhang X et al. Nucleolar and Spindle Associated Protein 1 (NUSAP1) Inhibits Cell Proliferation and Enhances Susceptibility to Epirubicin In Invasive Breast Cancer Cells by Regulating Cyclin D Kinase (CDK1) and DLGAP5 Expression. Med Sci Monit 24:8553-8564 (2018). PubMed: 30476929
- Espinoza I et al. Hypoxia on the Expression of Hepatoma Upregulated Protein in Prostate Cancer Cells. Front Oncol 6:144 (2016). IHC . PubMed: 27379206
- Ma MP et al. Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation. PLoS One 8:e68387 (2013). PubMed: 23861900