Anti-HURP 抗体 (ab70744)
Key features and details
- Rabbit polyclonal to HURP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HURP antibody
HURP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HURP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Gorilla
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免疫原
Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 846) of Human HURP (NP_055565.2).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70744の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 94 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 94 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. -
組織特異性
Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta. -
配列類似性
Belongs to the SAPAP family. -
発生段階
Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells. -
翻訳後修飾
Ubiquitinated, leading to its degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells. - Information by UniProt
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参照データベース
- Entrez Gene: 9787 Human
- SwissProt: Q15398 Human
- Unigene: 77695 Human
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別名
- DAP-5 antibody
- DAP5 antibody
- Discs large homolog 7 antibody
see all
画像
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Immunoprecipitation - Anti-HURP antibody (ab70744)Detection of HURP by Western Blot of Immunprecipitate. ab70744 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70744 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Chemiluminescence with exposure time of 10 seconds. -
Western blot - Anti-HURP antibody (ab70744)All lanes : Anti-HURP antibody (ab70744) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 94 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab70744 は 6 報の論文で使用されています。
- Luo K et al. Integrated bioinformatics analysis and experimental validation reveals hub genes of rheumatoid arthritis. Exp Ther Med 26:480 (2023). PubMed: 37745040
- Wu S et al. LINC01343 targets miR-526b-5p to facilitate the development of hepatocellular carcinoma by upregulating ROBO1. Sci Rep 13:17282 (2023). PubMed: 37828032
- Xiang Q et al. In vitro study of anti-ER positive breast cancer effect and mechanism of 1,2,3,4-6-pentyl-O-galloyl-beta-d-glucose (PGG). Biomed Pharmacother 111:813-820 (2019). PubMed: 30616080
- Si M et al. Integrated Analysis To Identify Molecular Biomarkers Of High-Grade Serous Ovarian Cancer. Onco Targets Ther 12:10057-10075 (2019). PubMed: 31819501
- Zhang Y et al. The microtubule-associated protein HURP recruits the centrosomal protein TACC3 to regulate K-fiber formation and support chromosome congression. J Biol Chem 293:15733-15747 (2018). PubMed: 30054275
- Möckel MM et al. Xenopus laevis Kif18A is a highly processive kinesin required for meiotic spindle integrity. Biol Open 6:463-470 (2017). PubMed: 28228376