Anti-Human Polyoma virus JCV capsid protein VP1 抗体 [8E8] (ab34756)
Key features and details
- Mouse monoclonal [8E8] to Human Polyoma virus JCV capsid protein VP1
- Suitable for: Indirect ELISA, WB
- Reacts with: JCV virus
- Isotype: IgG2a
製品の概要
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製品名
Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] -
製品の詳細
Mouse monoclonal [8E8] to Human Polyoma virus JCV capsid protein VP1 -
由来種
Mouse -
アプリケーション
適用あり: Indirect ELISA, WBmore details -
種交差性
交差種: JCV virus -
免疫原
Recombinant full length protein corresponding to Human Polyoma virus JCV capsid protein VP1. Recombinant full length purified major capsid protein VP1 of human polyomavirus JCV expressed in yeast S.cerevisiae.
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特記事項
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR48370-3, GR185137-5, GR185137-7 and GR185137-8 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
8E8 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34756の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Indirect ELISA |
1/1000 - 1/10000.
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WB |
1/1000 - 1/5000.
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特記事項 |
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Indirect ELISA
1/1000 - 1/10000. |
WB
1/1000 - 1/5000. |
ターゲット情報
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関連性
The human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins.
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プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab34756 は 18 報の論文で使用されています。
- Cheng Z et al. MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues. Adv Sci (Weinh) 10:e2302249 (2023). PubMed: 37658522
- Prezioso C et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. Viruses 14:N/A (2022). PubMed: 36146876
- Henriksen S & Rinaldo CH Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. Viruses 2022, 14, 2070. Viruses 15:N/A (2022). PubMed: 36680132
- Shen C et al. The differential presence of human polyomaviruses, JCPyV and BKPyV, in prostate cancer and benign prostate hypertrophy tissues. BMC Cancer 21:1141 (2021). PubMed: 34689739
- Wilczek MP et al. PI3K/AKT/mTOR Signaling Pathway Is Required for JCPyV Infection in Primary Astrocytes. Cells 10:N/A (2021). PubMed: 34831441