AB34756

Anti-Human Polyoma virus JCV capsid protein VP1 抗体 [8E8]

Name: Anti-Human Polyoma virus JCV capsid protein VP1 抗体 [8E8] (ab34756) | Abcam
Brand: Abcam Limited
SKU: AB34756
Price: 65000 JPY
Availability: InStock
Rating: 3 (2 reviews)

Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8]

(2 Reviews)

(18 Publications)

Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756) is a mouse monoclonal antibody detecting Human Polyoma virus JCV capsid protein VP1 in Western Blot, ELISA. Suitable for JCV virus.

- Over 10 publications
- Trusted since 2006

別名を表示する

Major capsid protein VP1, Major structural protein VP1

1 Images

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Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (AB34756)

Immunoblot of recombinant major capsid protein VP1 (500 ng per lane) of human polyomavirus JCV using monoclonal antibody ab34756 at a concentration of 1 μg/mL.

All lanes:

Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756)

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

8E8

アイソタイプ

IgG2a

キャリアフリー

交差種

JC polyomavirus

アプリケーション

WB, I-ELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "JC polyomavirus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant full length protein - JC polyomavirus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "1/1000 - 1/10000", "IELISA-species-notes": "<p></p>" } } }

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製品の詳細

What is this antibody validated in?
Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), ELISA in JCV virus samples.

Trusted by the scientific community
Anti-Human Polyoma virus JCV capsid protein VP1 [8E8] (ab34756) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with a N-linked glycoprotein containing terminal alpha(2-6)-linked sialic acids on the cell surface to provide virion attachment to target cell. The serotonergic receptor 5HT2AR also acts as a cellular receptor for JCV on human glial cells. Once attached, the virions enter predominantly by a ligand-inducible clathrin-dependent pathway and traffic to the ER. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA at nuclear domains called promyelocytic leukemia (PML) bodies, and participates in rearranging nucleosomes around the viral DNA.

See full target information VP1_POVJC

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302249 PubMed37658522

2023

MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangyu Cheng,Caroline Stefani,Thomas Skillman,Aleksandra Klimas,Aramchan Lee,Emma F DiBernardo,Karina Mueller Brown,Tatyana Milman,Yuhong Wang,Brendan R Gallagher,Katherine Lagree,Bhanu P Jena,Jose S Pulido,Scott G Filler,Aaron P Mitchell,N Luisa Hiller,Adam Lacy-Hulbert,Yongxin Zhao

Viruses 15: PubMed36680132

2022

Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. 2022, , 2070.

Applications

Unspecified application

Species

Unspecified reactive species

Stian Henriksen,Christine Hanssen Rinaldo

Viruses 14: PubMed36146876

2022

COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Prezioso,Sara Passerini,Dolores Limongi,Anna Teresa Palamara,Ugo Moens,Valeria Pietropaolo

Cells 10: PubMed34831441

2021

PI3K/AKT/mTOR Signaling Pathway Is Required for JCPyV Infection in Primary Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Wilczek,Francesca J Armstrong,Colleen L Mayberry,Benjamin L King,Melissa S Maginnis

BMC cancer 21:1141 PubMed34689739

2021

The differential presence of human polyomaviruses, JCPyV and BKPyV, in prostate cancer and benign prostate hypertrophy tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Chenghuang Shen,Chunliang Tung,Chunnun Chao,Yeongchin Jou,Shupei Huang,Menghsiao Meng,Deching Chang,Peilain Chen

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 128:104435 PubMed32442760

2020

Archetype JC polyomavirus DNA associated with extracellular vesicles circulates in human plasma samples.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano Scribano,Mirko Guerrini,Rosaria Arvia,Daniele Guasti,Patrizia Nardini,Paolo Romagnoli,Simone Giannecchini

Journal of virology 94: PubMed31826993

2020

JC Polyomavirus Infection Reveals Delayed Progression of the Infectious Cycle in Normal Human Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Wilczek,Jeanne K DuShane,Francesca J Armstrong,Melissa S Maginnis

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 20:1188-1191 PubMed31654479

2019

High-grade urothelial carcinoma in a kidney transplant recipient after JC virus nephropathy: The first evidence of JC virus as a potential oncovirus in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Querido,Isabel Fernandes,André Weigert,Sandra Casimiro,Catarina Albuquerque,Sância Ramos,Teresa Adragão,Ivan Luz,Paulo Paixão,Maria Chasqueira,Madalena Santos,Domingos Machado

Annals of neurology 86:878-884 PubMed31600832

2019

Fatal encephalopathy with wild-type JC virus and ruxolitinib therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Bowen Reoma,Christopher Julius Trindade,Maria Chiara Monaco,Jamie Solis,Marta Garcia Montojo,Phuong Vu,Kory Johnson,Erin Beck,Govind Nair,Omar I Khan,Marta Quezado,Stephen M Hewitt,Daniel S Reich,Richard Childs,Avindra Nath

Frontiers in microbiology 10:783 PubMed31065251

2019

High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jeanne K DuShane,Michael P Wilczek,Mason A Crocker,Melissa S Maginnis

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Anti-Human Polyoma virus JCV capsid protein VP1 抗体 [8E8]

Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8]

Western blot - Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (AB34756)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (18)

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