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AB324828

Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] - BSA and Azide free

Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] - BSA and Azide free

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Mouse Recombinant Monoclonal Minor capsid protein L2 antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Human papillomavirus 31, Recombinant fragment - Human papillomavirus 31 samples.

別名を表示する

Minor capsid protein L2

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Indirect ELISA - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] - BSA and Azide free (AB324828)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] - BSA and Azide free (AB324828)

This data was developed using ab322520, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322520 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : HPV Minor capsid protein L2.

Antigen concentration : 5000 ng/ml

Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] - BSA and Azide free (AB324828)
  • WB

Supplier Data

Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] - BSA and Azide free (AB324828)

This data was developed using ab322520, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)] (<a href='/products/primary-antibodies/human-papillomavirus-type-31-l2-antibody-k3hpv31l220-38-ab322520'>ab322520</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Human papillomavirus type 31 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with a Human papillomavirus type 33 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with a Human papillomavirus type 16 L2 protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 6s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-Human papillomavirus type 31 L2 antibody [K3_HPV31L2(20-38)]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

K3_HPV31L2(20-38)

アイソタイプ

IgG1

キャリアフリー

Yes

交差種

Human papillomavirus 31

アプリケーション

I-ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus 31": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Human papillomavirus 31": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Human papillomavirus 31": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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製品の詳細

ab324828 is the carrier-free version of ab322520.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, L2 escorts the genomic DNA into the nucleus by promoting escape from the endosomal compartments and traffic through the host Golgi network. Mechanistically, the C-terminus of L2 possesses a cell-penetrating peptide that protudes from the host endosome, interacts with host cytoplasmic retromer cargo and thereby mediates the capsid delivery to the host trans-Golgi network. Plays a role through its interaction with host dynein in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to host PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA.
See full target information L2
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Abcam product promise

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