Anti-Human papillomavirus L2 antibody [K18_HPV16L2(20-38)] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human papillomavirus L2 antibody [K18_HPV16L2(20-38)] - BSA and Azide free (AB324829)

This data was developed using ab322522, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus L2 protein with ab322522 at 1/100 (10.45 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a Human papillomavirus type 16 L2 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4μg/ml) dilution (Magenta).

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Human papillomavirus L2 antibody [K18_HPV16L2(20-38)] - BSA and Azide free (AB324829)

This data was developed using ab322522, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322522 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Human HPV16 L2 Protein.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-Human papillomavirus L2 antibody [K18_HPV16L2(20-38)] - BSA and Azide free (AB324829)

This data was developed using ab322522, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Human papillomavirus L2 antibody [K18_HPV16L2(20-38)] (<a href='/products/primary-antibodies/human-papillomavirus-l2-antibody-k18hpv16l220-38-ab322522'>ab322522</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Human papillomavirus type 16 L2 protein expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Human papillomavirus type 18 L2 protein expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Human papillomavirus type 31 L2 protein expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Human papillomavirus type 6B L2 protein expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a Human papillomavirus type 11 L2 protein expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 8s