AB109761

Anti-Human IgG3 抗体 [EPR4419]

Anti-Human IgG3 antibody [EPR4419]

RabMAb
Recombinant
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(11 Publications)

Rabbit Recombinant Monoclonal IGHG3 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 11 publications.

別名を表示する

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

1 Images

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Western blot - Anti-Human IgG3 antibody [EPR4419] (AB109761)

All lanes:

Western blot - Anti-Human IgG3 antibody [EPR4419] (ab109761) at 1/1000 dilution

Lane 1:

IgG3 at 0.01 µg

Lane 2:

IgG1 at 0.01 µg

Lane 3:

IgG2 at 0.01 µg

Lane 4:

IgG4 recombinant protein at 0.01 µg

Lane 5:

Human tonsil lysate at 10 µg

Lane 6:

Human plasma lysate

Predicted band size: 41 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4419

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab109761 does not cross-react with human IgG1, IgG2, and IgG4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Stable for 12 months at -20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGHG3

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of proteome research 21:274-288 PubMed34878788

2021

Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael D Melani,Benjamin J Des Soye,Jared O Kafader,Eleonora Forte,Michael Hollas,Voislav Blagojevic,Fernanda Negrão,John P McGee,Bryon Drown,Cameron Lloyd-Jones,Henrique S Seckler,Jeannie M Camarillo,Philip D Compton,Richard D LeDuc,Bryan Early,Ryan T Fellers,Byoung-Kyu Cho,Basil Baby Mattamana,Young Ah Goo,Paul M Thomas,Michelle K Ash,Pavan P Bhimalli,Lena Al-Harthi,Beverly E Sha,Jeffrey R Schneider,Neil L Kelleher

medRxiv : the preprint server for health sciences : PubMed34268518

2021

Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Applications

Unspecified application

Species

Unspecified reactive species

Frontiers in oncology 11:652835 PubMed34141611

2021

Silencing of the Long Non-Coding RNA TTN-AS1 Attenuates the Malignant Progression of Osteosarcoma Cells by Regulating the miR-16-1-3p/TFAP4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xianghai Meng,Zhenjun Zhang,Lin Chen,Xi Wang,Qingguo Zhang,Shuheng Liu

Molecular medicine reports 23: PubMed33760112

2021

MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Shuting Li,Yao Liu,Xujing Lv,Qing Zhou

Cancer cell international 21:73 PubMed33494763

2021

Hsa_circ_0010235 functions as an oncogenic drive in non-small cell lung cancer by modulating miR-433-3p/TIPRL axis.

Applications

Unspecified application

Species

Unspecified reactive species

Furui Zhang,Ruirui Cheng,Ping Li,Chunya Lu,Guojun Zhang

Cancer management and research 13:521-535 PubMed33505171

2021

Circ_0105346 Knockdown Inhibits Osteosarcoma Development via Regulating miR-1182/WNT7B Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinbao Liu,Xiaoyang Li,Liang Yue,Hao Lv

Cell cycle (Georgetown, Tex.) 19:3289-3302 PubMed33131409

2020

Depletion of circ_0007841 inhibits multiple myeloma development and BTZ resistance via miR-129-5p/JAG1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Quande Lin,Chunge Song,Ruojin Ma,Xiaojie Li

Molecular medicine reports 22:5095-5104 PubMed33174038

2020

Potential role of lncRNA HULC/miR‑128‑3p/RAC1 axis in the inflammatory response during LPS‑induced sepsis in HMEC‑1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Weize Yang,Xiaomin Luo,Yu Liu,Jun Xiong,Hongxia Xia,Yafeng Liu

Open life sciences 15:683-695 PubMed33817257

2020

CircHIPK3/miR-381-3p axis modulates proliferation, migration, and glycolysis of lung cancer cells by regulating the AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gu,Junhan Zhang,Lin Yan,Dong Li

Nature immunology 19:1001-1012 PubMed30104633

2018

IgG3 regulates tissue-like memory B cells in HIV-infected individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Lela Kardava,Haewon Sohn,Christine Youn,James W Austin,Wei Wang,Clarisa M Buckner,J Shawn Justement,Valerie A Melson,Gwynne E Roth,Marissa A Hand,Kathleen R Gittens,Richard W Kwan,Michael C Sneller,Yuxing Li,Tae-Wook Chun,Peter D Sun,Susan K Pierce,Susan Moir

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human IgG3 抗体 [EPR4419]

Anti-Human IgG3 antibody [EPR4419]

Western blot - Anti-Human IgG3 antibody [EPR4419] (AB109761)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (11)

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