AB181236

Anti-Human IgG 抗体 [EPR12700]

Anti-Human IgG antibody [EPR12700]

RabMAb
Recombinant
Lab Essentials
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(22 Publications)

Rabbit Recombinant Monoclonal IGHG1 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 22 publications.

別名を表示する

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (AB181236)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (AB181236)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (AB181236)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (AB181236)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Human IgG with purified ab181236 at 1/100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

Lab

Western blot - Anti-Human IgG antibody [EPR12700] (AB181236)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution

All lanes:

Human tonsil lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 52 kDa

false

Supplier Data

Western blot - Anti-Human IgG antibody [EPR12700] (AB181236)

All lanes:

Western blot - Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution

Lane 1:

Human IgG at 0.01 µg

Lane 2:

Human IgM at 0.01 µg

Lane 3:

Human IgA at 0.01 µg

Lane 4:

Human IgD at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-Human IgG antibody [EPR12700] (AB181236)

All lanes:

Western blot - Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human thymus lysate at 10 µg

Lane 3:

Human tonsil lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa

false

Lab

Western blot - Anti-Human IgG antibody [EPR12700] (AB181236)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution

All lanes:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 148 kDa,36 kDa,39 kDa,66 kDa,67 kDa,82 kDa,99 kDa

Observed band size: 110 kDa,149 kDa,52 kDa,55 kDa,66 kDa,82 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR12700

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ELISA, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.

See full target information IGHG1

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文献 (22)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1480461 PubMed39926286

2025

Mature tertiary lymphoid structure associated CD103+ CD8+ Trm cells determined improved anti-tumor immune in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Fang,Shuru Chen,Xiaoyue Chen,Wei Zou,Di Chen,Yukang Huang,Chucheng Wu

American journal of cancer research 14:5539-5550 PubMed39659936

2024

LncRNA SLC16A1-AS1 participates in the initiation and progression of colorectal cancer by regulating MAP3K9 expression through targeting miR-515-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Wanxing Xu,Suzhen Bi,Meichun Xing

Cancers 16: PubMed39001403

2024

Method for B Cell Receptor Enrichment in Malignant B Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Puja Bhattacharyya,Richard I Christopherson,Kristen K Skarratt,Stephen J Fuller

European journal of medical research 29:309 PubMed38831471

2024

Long non-coding RNA Small Nucleolar RNA Host Gene 4 ameliorates cigarette smoke-induced proliferation, apoptosis, inflammation, and airway remodeling in alveolar epithelial cells through the modulation of the mitogen-activated protein kinase signaling pathway via the microRNA-409-3p/Four and a Half LIM Domains 1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Liu,JiGuang Meng,XuXin Chen,Fan Wang,ZhiHai Han

iScience 27:109861 PubMed38799570

2024

Circ_0007432 promotes non-small cell lung cancer progression and macrophage M2 polarization through SRSF1/KLF12 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Mao,Dongyu Wu,Xiaozhen Cheng,Jinsheng Wu

European journal of medical research 29:67 PubMed38245787

2024

circNINL facilitates aerobic glycolysis, proliferation, invasion, and migration in lung cancer by sponging miR-3918 to mediate FGFR1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Li,Chun Qiu,DaTong Sun,ShengHui Yang,Lin Wang

Nature communications 14:8063 PubMed38052794

2023

Copper assisted sequence-specific chemical protein conjugation at a single backbone amide.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhun Guo,Kai Zhao,Liang Guo,Rui Zhou,Qiuju He,Kuan Lu,Tian Li,Dandan Liu,Jinfeng Chen,Jing Tang,Xin Fu,Jinyun Zhou,Bei Zheng,Samuel I Mann,Yongdeng Zhang,Jing Huang,Bing Yang,Ting Zhou,Yingjie Lei,Bobo Dang

Acta biochimica Polonica 70:777-784 PubMed37851953

2023

Circular RNA AGFG1 motivates breast cancer cell proliferation, invasion, migration, and glycolysis by controlling microRNA-653-5p/14-3-3 protein epsilon.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Chen,JinXian Qian,Ying Shen,Xiang Yu

Heliyon 9:e21056 PubMed37876483

2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting He,Huiyu Wang,Runjie Wang,Yuting Li,Suqing Li,Xiufeng Cao,Junying Xu

Acta biochimica Polonica 70:295-304 PubMed37220402

2023

Circular RNA-AnnexinA7 accelerates cisplatin resistance in non-small cell lung cancer via modulating microRNA-545-3p to mediate Cyclin D1.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yao,Hai Yang Zhang,Shuang Gu,Jin Long Zou,Qiang Zhang,Ri Chu Qu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human IgG 抗体 [EPR12700]

Anti-Human IgG antibody [EPR12700]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (AB181236)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (AB181236)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] (AB181236)

Western blot - Anti-Human IgG antibody [EPR12700] (AB181236)

Western blot - Anti-Human IgG antibody [EPR12700] (AB181236)

Western blot - Anti-Human IgG antibody [EPR12700] (AB181236)

Western blot - Anti-Human IgG antibody [EPR12700] (AB181236)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (22)

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