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リコンビナントRabMAb

Anti-HtrA2 / Omi 抗体 [EPR22] (ab75982)

Reviews (2) Submit a question References (10)
Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
  • Flow Cytometry (Intracellular) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
  • Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
  • Anti-HtrA2 / Omi antibody [EPR22] (ab75982)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22] to HtrA2 / Omi
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-HtrA2 / Omi antibody [EPR22]
    HtrA2 / Omi 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22] to HtrA2 / Omi

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human HtrA2/ Omi.

  • ポジティブ･コントロール

    • WB: Jurkat, 293T, and HeLa cell lysates. IHC-P: Human colon tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75982の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (2)
1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/500 - 1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.

  • 組織特異性

    Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

  • 関連疾患

    Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.

  • 配列類似性

    Belongs to the peptidase S1B family.
    Contains 1 PDZ (DHR) domain.

  • ドメイン

    The mature N-terminus is involved in the interaction with XIAP.
    The PDZ domain mediates interaction with MXI2.

  • 翻訳後修飾

    Autoproteolytically activated.

  • 細胞内局在

    Mitochondrion intermembrane space. Mitochondrion membrane. Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.
  • Information by UniProt

  • 参照データベース

    • 別名

      • High temperature requirement protein A2 antibody
      • HTRA 2 antibody
      • HtrA like serine protease antibody
      • HtrA serine peptidase 2 antibody
      • HtrA, E. coli, homolog of, 2 antibody
      • HtrA2 antibody
      • HTRA2_HUMAN antibody
      • mitochondrial antibody
      • Omi stress regulated endoprotease antibody
      • Omi stress-regulated endoprotease antibody
      • PARK 13 antibody
      • PARK13 antibody
      • Protease serine 25 antibody
      • PRSS 25 antibody
      • PRSS25 antibody
      • Serine protease 25 antibody
      • Serine protease HTRA2 antibody
      • Serine protease HTRA2 mitochondrial antibody
      • Serine protease htra2 mitochondrial precursor antibody
      • Serine protease omi antibody
      • Serine proteinase OMI antibody
      see all

    画像

    • Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
      Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
      All lanes : Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 49 kDa
      Observed band size: 38 kDa why is the actual band size different from the predicted?
      Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.



      Secondary antibody - goat anti-rabbit HRP (ab6721)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)

      Immunohistochemical analysis of paraffin-embedded human colon using ab75982 at 1/100-1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
      Flow Cytometry (Intracellular) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)

      Overlay histogram showing HeLa cells stained with ab75982 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75982, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluor® 488 (IgG H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

       

       

    • Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
      Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)This image is courtesy of an abreview submitted by Christian Marx
      Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/1000 dilution + Human Isolated mitochondria from NB4 cell line at 30 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking buffer : 5% milk

      Diluting buffer : 1% milk

      High background signal after longer exposure.

      See Abreview

    • Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
      Anti-HtrA2 / Omi antibody [EPR22] (ab75982)

    参考文献 (10)

    ab75982 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab75982 は 10 報の論文で使用されています。

    • Yu X  et al. Apoptin causes apoptosis in HepG-2 cells via Ca2+ imbalance and activation of the mitochondrial apoptotic pathway. Cancer Med 12:8306-8318 (2023). PubMed: 36515089
    • Weiß J  et al. Protease-independent control of parthanatos by HtrA2/Omi. Cell Mol Life Sci 80:258 (2023). PubMed: 37594630
    • Zhou J  et al. Yap-Hippo promotes A549 lung cancer cell death via modulating MIEF1-related mitochondrial stress and activating JNK pathway. Biomed Pharmacother 113:108754 (2019). PubMed: 30875659
    • Zhang C  et al. Inhibition of HtrA2 alleviated dextran sulfate sodium (DSS)-induced colitis by preventing necroptosis of intestinal epithelial cells. Cell Death Dis 10:344 (2019). PubMed: 31019191
    • Shen Y  et al. Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice. Front Cell Neurosci 13:568 (2019). PubMed: 31969805
    • Zhang L  et al. Regulation of HtrA2 on WT1 gene expression under imatinib stimulation and its effects on the cell biology of K562 cells. Oncol Lett 14:3862-3868 (2017). PubMed: 28927158
    • Liu R  et al. A New Perspective for Osteosarcoma Therapy: Proteasome Inhibition by MLN9708/2238 Successfully Induces Apoptosis and Cell Cycle Arrest and Attenuates the Invasion Ability of Osteosarcoma Cells in Vitro. Cell Physiol Biochem 41:451-465 (2017). PubMed: 28214890
    • Miyamoto M  et al. High-temperature-required protein A2 as a predictive marker for response to chemotherapy and prognosis in patients with high-grade serous ovarian cancers. Br J Cancer 112:739-44 (2015). IHC-P ; Human . PubMed: 25628093
    • Mihailovich M  et al. miR-17-92 fine-tunes MYC expression and function to ensure optimal B cell lymphoma growth. Nat Commun 6:8725 (2015). WB ; Mouse . PubMed: 26555894
    • Rami A  et al. Translocation of the serine protease Omi/HtrA2 from mitochondria into the cytosol upon seizure-induced hippocampal injury in the neonatal rat brain. Neurochem Res 35:2199-207 (2010). IHC-P ; Rat . PubMed: 21132459

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-HtrA2 / Omi antibody [EPR22]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 11 2015

    Western blot abreview for Anti-HtrA2 / Omi antibody [EPR22]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (isolated mitochondria from NB4 cell line)
    Loading amount
    30 µg
    Specification
    isolated mitochondria from NB4 cell line
    Gel Running Conditions
    Reduced Denaturing (13%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Christian Marx

    Verified customer

    投稿 Feb 15 2013

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