Anti-HtrA2 / Omi 抗体 [E55] (ab32092)
![Western blot - Anti-HtrA2 / Omi antibody [E55] (ab32092)](https://www.abcam.co.jp/ps/products/32/ab32092/Images/ab32092-43861-anti-htra2-omi-antibody-e55-western-blot.jpg "Western blot - Anti-HtrA2 / Omi antibody [E55] (ab32092)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E55] to HtrA2 / Omi
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HtrA2 / Omi antibody [E55]
HtrA2 / Omi 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E55] to HtrA2 / Omi -
由来種
Rabbit -
特異性
ab32092 does not cross-react with other peptidase S1C family members. -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human HtrA2/ Omi aa 100-200 (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- Jurkat cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E55 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32092の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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| IHC-P |
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
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IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
追加情報
Is unsuitable for Flow Cyt or ICC/IF.
ターゲット情報
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機能
Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. -
組織特異性
Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid. -
関連疾患
Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. -
配列類似性
Belongs to the peptidase S1B family.
Contains 1 PDZ (DHR) domain. -
ドメイン
The mature N-terminus is involved in the interaction with XIAP.
The PDZ domain mediates interaction with MXI2. -
翻訳後修飾
Autoproteolytically activated. -
細胞内局在
Mitochondrion intermembrane space. Mitochondrion membrane. Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID. - Information by UniProt
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参照データベース
- Entrez Gene: 27429 Human
- Entrez Gene: 64704 Mouse
- Entrez Gene: 297376 Rat
- Omim: 606441 Human
- SwissProt: O43464 Human
- SwissProt: Q9JIY5 Mouse
- Unigene: 469045 Human
- Unigene: 21880 Mouse
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別名
- High temperature requirement protein A2 antibody
- HTRA 2 antibody
- HtrA like serine protease antibody
see all
画像
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Western blot - Anti-HtrA2 / Omi antibody [E55] (ab32092)Anti-HtrA2 / Omi antibody [E55] (ab32092) at 1/2000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 38 kDa
Observed band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [E55] (ab32092)](https://www.abcam.co.jp/ps/products/32/ab32092/Images/ab32092-43860-anti-htra2-omi-antibody-e55-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [E55] (ab32092)ab32092 at a 1:250 dilution staining Htr2A/ Omi in human lung adenocarcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-HtrA2 / Omi antibody [E55] (ab32092)](https://www.abcam.co.jp/ps/products/32/ab32092/Images/ab32092-3-benefits-of-recombinant-antibodies.png)
Anti-HtrA2 / Omi antibody [E55] (ab32092)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab32092 は 17 報の論文で使用されています。
- Cho N et al. A novel splicing variant of DJ-1 in Parkinson's disease induces mitochondrial dysfunction. Heliyon 9:e14039 (2023). PubMed: 36915530
- Zhou W et al. HtrA2/Omi mitigates NAFLD in high-fat-fed mice by ameliorating mitochondrial dysfunction and restoring autophagic flux. Cell Death Discov 8:218 (2022). PubMed: 35449197
- Wang X Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide-induced acute pneumonia in rats. Mol Med Rep 22:3127-3134 (2020). PubMed: 32945404
- Ouyang H et al. Inhibitory effect of melatonin on Mst1 ameliorates myocarditis through attenuating ER stress and mitochondrial dysfunction. J Mol Histol 50:405-415 (2019). PubMed: 31256303
- He M et al. Lipopolysaccharide induces human olfactory ensheathing glial apoptosis by promoting mitochondrial dysfunction and activating the JNK-Bnip3-Bax pathway. Cell Stress Chaperones 24:91-104 (2019). PubMed: 30374881