Anti-HSV1 ICP8 Major DNA binding protein 抗体 [10A3] (ab20193)
![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (ab20193)](https://www.abcam.co.jp/ps/products/20/ab20193/Images/ab20193-212420-anti-hsv1-icp8-major-dna-binding-protein-antibody-10a3-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (ab20193)")
Key features and details
- Mouse monoclonal [10A3] to HSV1 ICP8 Major DNA binding protein
- Suitable for: WB, ICC/IF
- Reacts with: Herpes simplex virus
- Isotype: IgG1
製品の概要
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製品名
Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3]
HSV1 ICP8 Major DNA binding protein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [10A3] to HSV1 ICP8 Major DNA binding protein -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Herpes simplex virus -
免疫原
ICP8 purified from U-35-VERO cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
10A3 -
ミエローマ
Sp2 -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20193の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.
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| ICC/IF | (1) |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
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WB
Use at an assay dependent dilution. Predicted molecular weight: 129 kDa. |
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ICC/IF
Use at an assay dependent dilution. |
ターゲット情報
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関連性
Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. ICP8 is the major DNA binding protein of herpes simplex virus type 1. -
参照データベース
- Entrez Gene: 2703458 Herpes simplex virus
- SwissProt: P04296 Herpes simplex virus
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別名
- DBP antibody
- Herpes simplex virus type 1 DBP antibody
- HSV1 DBP antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (ab20193)This image is courtesy of an anonymous Abreview
ab20193 staining HSV1 ICP8 Major DNA binding protein in Human U2OS by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab20193 は 11 報の論文で使用されています。
- Meier AF et al. Herpes Simplex Virus 1 Coinfection Modifies Adeno-associated Virus Genome End Recombination. J Virol 95:e0048621 (2021). PubMed: 33853961
- Vink EI et al. Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34725147
- Crameri M et al. MxB is an interferon-induced restriction factor of human herpesviruses. Nat Commun 9:1980 (2018). PubMed: 29773792
- Valledor M et al. Herpes ICP8 protein stimulates homologous recombination in human cells. PLoS One 13:e0200955 (2018). PubMed: 30110337
- Franzoso FD et al. Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1. J Virol 91:N/A (2017). PubMed: 28515305