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Anti-HSV1 gC Envelope Protein 抗体 [3G9] (ab6509)

Reviews (2)Q&A (3)References (14)
Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)

    Key features and details

    • Mouse monoclonal [3G9] to HSV1 gC Envelope Protein
    • Suitable for: WB, ICC/IF
    • Reacts with: Herpes simplex virus
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-HSV1 gC Envelope Protein antibody [3G9]

    • 製品の詳細

      Mouse monoclonal [3G9] to HSV1 gC Envelope Protein

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WB, ICC/IFmore details

    • 種交差性

      交差種: Herpes simplex virus

    • 免疫原

      Tissue, cells or virus corresponding to Herpes simplex virus HSV1 gC Envelope Protein. Herpes Virus

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.4
    • Concentration information loading...

    • 精製度

      Protein A purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      3G9

    • ミエローマ

      NS1/1-Ag4-1

    • アイソタイプ

      IgG2a

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6509の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    1/1000.
    ICC/IF
    1/25600.
    特記事項
    WB
    1/1000.
    ICC/IF
    1/25600.

    ターゲット情報

    • 関連性

      Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell.

    • 参照データベース

      • 別名

        • Envelope glycoprotein C antibody
        • GC antibody
        • Glycoprotein C antibody
        • Herpes simplex virus 1 glycoprotein C antibody
        • UL44 antibody
        see all

      画像

      • Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)
        Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)Image from Livingston CM et al., PLoS Pathog. 2009 Oct;5(10):e1000619. Epub 2009 Oct 9.Fig 1.; doi:10.1371/journal.ppat.1000619; October 9, 2009, PLoS Pathog 5(10): e1000619.
        Western blot analysis of Vero cells mock-infected or infected with KOS at a multiplicity of infection of 10 for 1, 3, 6 and 9 hours.

        HSV1 gC Envelope Protein was detected using ab6509, at 1/1000 dilution. An HRP-conjugated sheep-anti-mouse IgG (1/20000) was used as the secondary antibody.

      参考文献 (14)

      ab6509 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6509 は 14 報の論文で使用されています。

      • Salgado B  et al. Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells. Microorganisms 11:N/A (2023). PubMed: 37317179
      • DiScipio KA  et al. Two-Metal Ion-Dependent Enzymes as Potential Antiviral Targets in Human Herpesviruses. mBio 13:e0322621 (2022). PubMed: 35073739
      • Murray-Nerger LA  et al. Lamin B1 acetylation slows the G1 to S cell cycle transition through inhibition of DNA repair. Nucleic Acids Res 49:2044-2064 (2021). PubMed: 33533922
      • Kristen H  et al. LAMP2 deficiency attenuates the neurodegeneration markers induced by HSV-1 infection. Neurochem Int 146:105032 (2021). PubMed: 33781848
      • Llorente P  et al. Matrix metalloproteinase 14 regulates HSV-1 infection in neuroblastoma cells. Antiviral Res 192:105116 (2021). PubMed: 34107282
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-HSV1 gC Envelope Protein antibody [3G9]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      African Green Monkey Cell lysate - whole cell (Vero cells)
      Loading amount
      10 µg
      Specification
      Vero cells
      Treatment
      Infected with HSV1 (KOS strain)
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 05 2007

      Western blot abreview for Anti-HSV1 gC Envelope Protein antibody [3G9]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa cells)
      Loading amount
      10 µg
      Specification
      HeLa cells
      Treatment
      Infected with HSV KOS
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Aug 28 2007

      Question

      what part of the protein is this antibody directed against?

      Read More
      Answer

      Thank you for your inquiry.  Unfortunately this antibody has not been epitope mapped, so we do not have any further information on where the antibody binds. Please do not hesitate to contact us if you need anything further.  Best of luck in your research.

      Read More

      Question

      Inquiry: Hello I would like to know if these monoclonal mouse antibodies ab0656 and ab6509 directed against HSV-1 gB and HSV-1 gC, Have been used to detect infected neurons in culture. Or If they can work to identify Infected neurons in histological preparations?. And what kind of fixation substance (PFA or Methanol) shoul I use to fix my neuron cells?

      Read More
      Answer

      Thank you very much for your interest in ab6506 and ab6509.

      To our knowledge, these products have not been tested inneurons in culture. Therefore, I can offer a discount off a future purchase if you buy one or both of these products now, test it in ICC on neurons and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY OR VALUE OFF OF NEXT ORDER for each antibody you test

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test one or both of these products in ICC on neurons. I will then send a discount code. These codes must be issued before purchasing so please wait for my reply before ordering.

      2. Purchase either by phone, fax, or online (www.abcam.com).

      3. Test it in neurons.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. Each discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product turns out to be unsuitable for this application, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Question

      Do these antibodies recognize the N-terminus or C-terminus of the protein?

      Read More
      Answer

      I apologize for the delay, but I just heard back for certain that this antibody has not been epitope mapped.  So neither ab6509 nor ab33658 have been mapped. I'm sorry that this information could not be more helpful.  Please contact us with any other questions.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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