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Anti-HSV1 + HSV2 VP16 抗体 [LP1] (ab110226)

Submit a review Q&A (1)References (26)
Western blot - Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226)
  • Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226)

Key features and details

  • Mouse monoclonal [LP1] to HSV1 + HSV2 VP16
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Herpes simplex virus, Human herpesvirus 2
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-HSV1 + HSV2 VP16 antibody [LP1]

  • 製品の詳細

    Mouse monoclonal [LP1] to HSV1 + HSV2 VP16

  • 由来種

    Mouse

  • 特異性

    This antibody is specific for Herpes simplex virus tegument protein VP16. This antibody will cross react with HSV1 and HSV2.

  • アプリケーション

    適用あり: ICC/IF, IP, WBmore details

  • 種交差性

    交差種: Herpes simplex virus, Human herpesvirus 2

  • 免疫原

    Tissue, cells or virus corresponding to HSV1 + HSV2 VP16. HSV-2.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab110226の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
IP
1/50.
WB
1/1000. Predicted molecular weight: 56 kDa.
特記事項
ICC/IF
1/100.
IP
1/50.
WB
1/1000. Predicted molecular weight: 56 kDa.

ターゲット情報

  • 参照データベース

    • 別名

      • Herpes simplex virus tegument protein VP16 antibody
      • HSV antibody
      • VP16 antibody

    画像

    • Western blot - Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226)
      Western blot - Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226)
      Vero cells were mock infected or infected with HSV-1 (strain SC16) at 10PFU/cell and harvested at 18h post infection. Cells were lysed in 50mM Tris pH 7.6, 150 mM NaCl, 1% sodium deoxycholate, 1% Triton X-100 supplemented with protease inhibitors. Samples were boiled in SDS-PAGE sample buffer, separated on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane by western blot. Membranes were blocked in PBS, 0.05% Tween-20, 5% dried milk powder and incubated with ab110226 at various dilutions followed by anti-mouse-IRdye 800CW and scanned using an infrared imager. Each lane corresponds to approximately 3x10^5 cells.
    • Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226)
      Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226)
      HeLa cells were infected with HSV-1 (strain SC16) at 3 PFU/cell and fixed in 4% formaldehyde at 18h post infection. Cells were permeabilised with 0.1% Triton X-100 and incubated with ab110226 (red) and anti-capsid (green) followed by Alexa-488 and Alexa-633 conjugated secondary antibodies.

    参考文献 (26)

    ab110226 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab110226 は 26 報の論文で使用されています。

    • Santos VC  et al. Slug, a Stress-Induced Transcription Factor, Stimulates Herpes Simplex Virus 1 Replication and Transactivates a cis-Regulatory Module within the VP16 Promoter. J Virol 97:e0007323 (2023). PubMed: 37022165
    • Suryawanshi RK  et al. Pathophysiology of reinfection by exogenous HSV-1 is driven by heparanase dysfunction. Sci Adv 9:eadf3977 (2023). PubMed: 37115924
    • Bhattacharya I  et al. OPTN-TBK1 axis and a role for PLK1 in HSV-1 infection. mBio 14:e0271523 (2023). PubMed: 38019030
    • Osaka R  et al. VP26, a herpes simplex virus type 1 capsid protein, increases DNA methylation in COASY promoter region. Brain Behav Immun Health 26:100545 (2022). PubMed: 36345321
    • Ames J  et al. OPTN is a host intrinsic restriction factor against neuroinvasive HSV-1 infection. Nat Commun 12:5401 (2021). PubMed: 34518549
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Please send an AbTrial code for testing ab110226 for detection of herpes B virus by western blotting.

    Read More
    Answer

    DISCOUNT CODE: ***
    Expiration date: ***

    I am very pleased to hear you would like to accept our offer and test ab110226 on blots of Herpes B virus. To redeem this offer, please submit an Abreview for Herpes B virus western blotting and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. When filling out the review form, look for "SHBV" or Herpes B virus in the list of species.

    We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com