Anti-HSPA1L 抗体 (ab154409)
Key features and details
- Rabbit polyclonal to HSPA1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HSPA1L antibody
HSPA1L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HSPA1L -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig, Xenopus tropicalis
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免疫原
Recombinant fragment corresponding to a region within amino acids 11-278 of Human HSPA1L (Uniprot ID P34931).
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ポジティブ･コントロール
- Recombinant Human HSPA1L protein (ab114801) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A540 cells; Human H661 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. -
組織特異性
Expressed in spermatids. -
配列類似性
Belongs to the heat shock protein 70 family. - Information by UniProt
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参照データベース
- Entrez Gene: 540190 Cow
- Entrez Gene: 3305 Human
- Entrez Gene: 15482 Mouse
- Entrez Gene: 100144518 Pig
- Entrez Gene: 24963 Rat
- Entrez Gene: 779883 Xenopus tropicalis
- Omim: 140559 Human
- SwissProt: P0CB32 Cow
see all -
別名
- Heat shock 70 kDa protein 1 Hom antibody
- Heat shock 70 kDa protein 1 like antibody
- Heat shock 70 kDa protein 1-Hom antibody
see all
画像
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Western blot - Anti-HSPA1L antibody (ab154409)Anti-HSPA1L antibody (ab154409) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154409)Immunofluorescence analysis of methanol-fixed A549 cells, labeling HSPA1L using ab154409 at a 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (ab154409)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human H661 xenograft tissue, labeling HSPA1L using ab154409 at a 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab154409 は 5 報の論文で使用されています。
- Liu X et al. Expressions of HSPA1L and HSPA9 are associated with poor sperm quality of low-motility spermatozoa in fertile men. Andrologia 54:e14321 (2022). PubMed: 34796524
- Nakatsuka A et al. A Vaspin-HSPA1L complex protects proximal tubular cells from organelle stress in diabetic kidney disease. Commun Biol 4:373 (2021). PubMed: 33742129
- Panagi I et al. Salmonella Effector SteE Converts the Mammalian Serine/Threonine Kinase GSK3 into a Tyrosine Kinase to Direct Macrophage Polarization. Cell Host Microbe 27:41-53.e6 (2020). PubMed: 31862381
- Liu T et al. Genomewide identification and analysis of heat-shock proteins 70/110 to reveal their potential functions in Chinese soft-shelled turtle Pelodiscus sinensis. Ecol Evol 9:6968-6985 (2019). PubMed: 31467669
- Zhou Y et al. Simultaneous generation of multi-gene knockouts in human cells. FEBS Lett 590:4343-4353 (2016). PubMed: 27800615