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遺伝子ノックアウト細胞株 検証済

Anti-Hsp90 beta 抗体 [K3701] (ab82584)

Submit a review Q&A (1)
Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)
  • Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)
  • Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [K3701] (ab82584)

Key features and details

  • Mouse monoclonal [K3701] to Hsp90 beta
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Rat, Human, Chinese hamster
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-Hsp90 beta antibody [K3701]
    Hsp90 beta 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [K3701] to Hsp90 beta

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details

  • 種交差性

    交差種: Rat, Human, Chinese hamster

  • 免疫原

    Recombinant human Hsp90 beta protein

  • ポジティブ･コントロール

    • WB: A431, Saos-2, HL-60, HeLa, PC12, CHO-K1 cell lysates; Hsp90 native protein. Flow Cyt (Intra): Human colon cancer Caco-2 cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Ammonium Sulphate Precipitation

  • ポリ/モノ

    モノクローナル

  • クローン名

    K3701

  • アイソタイプ

    IgM

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab82584の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 83 kDa.

1/1000 (colorimetric).
特記事項
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 83 kDa.

1/1000 (colorimetric).

ターゲット情報

  • 機能

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

  • 配列類似性

    Belongs to the heat shock protein 90 family.

  • ドメイン

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.

  • 翻訳後修飾

    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.

  • 細胞内局在

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 90 kda heat shock protein beta HSP90 beta antibody
      • D6S182 antibody
      • FLJ26984 antibody
      • Heat shock 84 kDa antibody
      • Heat shock 90kD protein 1, beta antibody
      • Heat shock 90kDa protein 1 beta antibody
      • Heat shock protein 90 alpha family class B member 1 antibody
      • Heat shock protein 90 kDa antibody
      • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
      • Heat shock protein 90kDa alpha family class B member 1 antibody
      • Heat shock protein beta antibody
      • Heat shock protein HSP 90 beta antibody
      • Heat shock protein HSP 90-beta antibody
      • HS90B_HUMAN antibody
      • HSP 84 antibody
      • HSP 90 antibody
      • HSP 90 b antibody
      • HSP 90b antibody
      • HSP84 antibody
      • HSP90 BETA antibody
      • hsp90ab1 antibody
      • HSP90B antibody
      • HSPC2 antibody
      • HSPCB antibody
      see all

    画像

    • Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)
      Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)
      All lanes : Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution

      Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 2 : HSP90AB1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : Saos-2 (Human osteosarcoma cell line) whole cell lysate
      Lane 4 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 83 kDa
      Observed band size: 85 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab82584 observed at 85 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.

      ab82584 was shown to react with Hsp90 beta in wild-type A431 cells in western blot. Loss of signal was observed when HSP90AB1 knockout sample was used. Wild-type A431 and HSP90AB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab82584 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)
      Western blot - Anti-Hsp90 beta antibody [K3701] (ab82584)
      All lanes : Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : CHO-K1 cell lysate
      Lane 4 : Hsp90 Native Protein
      Lane 5 : Hsp90 alpha Recombinant Protein (negative control)

      Predicted band size: 83 kDa
      Observed band size: 83 kDa

    • Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [K3701] (ab82584)
      Flow Cytometry (Intracellular) - Anti-Hsp90 beta antibody [K3701] (ab82584)
      ab82584, at 10 µg/ml, staining p90 beta in human colon cancer Caco-2 cells by flow cytometry (right). Isotype control antibody (left).

    参考文献 (0)

    ab82584 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab82584 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Doe this antibody recognize an intra-or extracellular epitope. Also can you provide the protocol that was used to test this antibody in flow cytometry.

    Read More
    Answer

    Thank you for your patience in this matter while I was in contact with the lab,

    I have attached the intracellular protocol used to test this antibody for Flow cytometry.

    If there is anything else I can help you with, please let me know.

    Read More

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