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Anti-Hsp60 抗体 [LK-2] (ab59458)

Reviews (1) Submit a question References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
  • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
  • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

Key features and details

  • Mouse monoclonal [LK-2] to Hsp60
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Hsp60 antibody [LK-2]
    Hsp60 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [LK-2] to Hsp60

  • 由来種

    Mouse

  • 特異性

    Detects ~60kDa.

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Human Hsp60 produced through recombinant DNA methods in E.coli

  • ポジティブ･コントロール

    • HeLa cell lysate

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab59458の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100.
WB (1)
1/40000. Predicted molecular weight: 60 kDa.
特記事項
IHC-P
1/100.
WB
1/40000. Predicted molecular weight: 60 kDa.

ターゲット情報

  • 機能

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

  • 関連疾患

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.

  • 配列類似性

    Belongs to the chaperonin (HSP60) family.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 60 kDa chaperonin antibody
      • 60 kDa heat shock protein, mitochondrial antibody
      • CH60_HUMAN antibody
      • Chaperonin 60 antibody
      • Chaperonin, 60-KD antibody
      • CPN60 antibody
      • fa04a05 antibody
      • GROEL antibody
      • heat shock 60kDa protein 1 (chaperonin) antibody
      • Heat shock protein 1 (chaperonin) antibody
      • Heat shock protein 60 antibody
      • Heat shock protein 65 antibody
      • heat shock protein family D (Hsp60) member 1 antibody
      • HLD4 antibody
      • Hsp 60 antibody
      • HSP 65 antibody
      • HSP-60 antibody
      • HSP60 antibody
      • HSP65 antibody
      • HSPD1 antibody
      • HuCHA60 antibody
      • Mitochondrial matrix protein P1 antibody
      • P60 lymphocyte protein antibody
      • short heat shock protein 60 Hsp60s1 antibody
      • SPG13 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

      Immunohistochemistry analysis of  Paraffin Embedded Human kidney tissue using Mouse Anti-HSP60 Antibody, Clone LK2 (SMC-111) at 1/100 dilution.

       

    • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
      Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
      Anti-Hsp60 antibody [LK-2] (ab59458) at 1/1000 dilution + HeLa cell lysate

      Predicted band size: 60 kDa

    • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
      Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)This image is courtesy of an anonymous Abreview
      Lanes 1-3 : Anti-Hsp60 antibody [LK-2] (ab59458) at 1/4000 dilution


      Lanes 1-3 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
      Lane 4 : Ladder

      Secondary
      Lanes 1-2 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution
      Lane 3 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution


      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 60 kDa


      Exposure time: 25 seconds

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

      ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

      Paraffin-embedded mouse back skin tissue stained for Hsp60 using ab59458 at 1/100 dilution in immunohistochemical analysis. DAB staining.

    参考文献 (6)

    ab59458 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab59458 は 6 報の論文で使用されています。

    • Jiang X  et al. Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N2-fixation in plants. Commun Biol 4:4 (2021). PubMed: 33398015
    • Senger F  et al. Spatial integration of mechanical forces by a-actinin establishes actin network symmetry. J Cell Sci 132:N/A (2019). PubMed: 31615968
    • Farrugia G  et al. Aspirin impairs acetyl-coenzyme A metabolism in redox-compromised yeast cells. Sci Rep 9:6152 (2019). PubMed: 30992471
    • Schultz MJ  et al. Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures. J Virol 91:N/A (2017). PubMed: 28446677
    • Mion F  et al. Mast cells control the expansion and differentiation of IL-10-competent B cells. J Immunol 193:4568-79 (2014). PubMed: 25267976
    • Bivi N  et al. Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates. J Proteome Res 8:1131-42 (2009). PubMed: 19226166

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-Hsp60 antibody [LK-2]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    6.9e+006 cells
    Gel Running Conditions
    Reduced Denaturing (4-12 % Bis-Tris, MOPS running buffer, semi-dry transfer to nitrocellulose)
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (W303alpha)
    Specification
    W303alpha
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    投稿 Apr 25 2014

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