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AB190828

Anti-Hsp60 抗体 [EPR18245-93]

Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker

4

(4 Reviews)

|

(34 Publications)

Anti-Hsp60 antibody [EPR18245-93] (ab190828) is a rabbit monoclonal antibody detecting Hsp60 in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

別名を表示する

HSP60, HSPD1, 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, CPN60, HSP-60, Hsp60

10 Images
Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Hsp60 with ab190828 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.

The nuclear counter stain is DAPI (blue). COX IV is detected with Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) (red) at 1/1000 dilution.

The negative controls are as follows : -

-ve control 1 : ab190828 at 1/200 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution.

-ve control 2 : Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Hsp60 with ab190828 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Hsp60 with ab190828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular and cytoplasmic staining on human liver (PMID : 18548335). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embyro fibroblast cell line) cell line labeling Hsp60 with ab190828 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embyro fibroblast cell line) cells labeling Hsp60 with ab190828 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Hsp60 with ab190828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular and cytoplasmic staining on rat liver (PMID : 18548335). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • IP

Supplier Data

Immunoprecipitation - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Hsp60 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embyro fibroblast cell line) cell lysate with ab190828 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190828 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : NIH/3T3 cell lysate 10 μg (Input).

Lane 2 : ab190828 IP in NIH/3T3 cell lysate .

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190828 in NIH/3T3 cell lysate .

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (ab190828)

Predicted band size: 61 kDa

false

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • WB

Supplier Data

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Exposure time : Lanes 1,2 and 3 : 3 minutes; Lane 4 : 1 minute.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (ab190828) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

true

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • WB

Supplier Data

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Exposure time : Lanes 1,2 and 3 : 30 seconds; Lane 4 : 15 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (ab190828) at 1/5000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma cell line) cell lysate at 20 µg

Lane 4:

C6 (rat glial tumor cell line) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

true

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)
  • WB

Supplier Data

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (AB190828)

Exposure time : Lane 1 : 30 seconds; Lane 2 : 1 minute; Lanes 3,4 and 5 : 10 seconds; Lane 6 : 5 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker (ab190828) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Lane 4:

Rat heart lysate at 10 µg

Lane 5:

Rat spleen lysate at 10 µg

Lane 6:

Rat liver lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

true

関連する標識済み抗体及び組成の異なる製品 (4)

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  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Hsp60 antibody [EPR18245-93] - Mitochondrial Marker

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18245-93

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB, IP, ICC/IF, Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

製品の詳細

What is this antibody validated in?
Anti-Hsp60 antibody [EPR18245-93] (ab190828) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Hsp60?
Anti-Hsp60 [EPR18245-93] (ab190828) specifically detects a band for Hsp60 (UniProt: P63038) at a molecular weight of 61kDa.

Trusted by the scientific community
Anti-Hsp60 [EPR18245-93] (ab190828) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Hsp60 also known as HSPD1 and heat shock protein 60 is a significant chaperonin with a molecular weight of approximately 60 kDa. It resides predominantly in the mitochondria and functions to assist in the proper folding of proteins preventing their aggregation. Hsp60 is expressed mainly in cells with high metabolic activity. Its presence as a mitochondrial marker highlights its essential role in maintaining cellular homeostasis and function.
Biological function summary

The protein ensures mitochondrial protein stability by facilitating the refolding of misfolded proteins and cooperating with other chaperonins like Hsp10. Hsp60 participates in forming a complex with these proteins to create a conducive environment for protein folding. It plays a part in regulating mitochondrial homeostasis impacting cell survival and apoptosis processes.

Pathways

Hsp60 links to the ATP synthesis and apoptosis pathways showcasing its importance as a mitochondrial marker. It interacts with proteins like caspase-3 to modulate cell death mechanisms highlighting its influence beyond simple protein folding. In the ATP synthesis pathway it contributes indirectly to energy production by maintaining mitochondrial function.

Hsp60 shows a connection to neurodegenerative diseases and cancer. Altered Hsp60 levels correlate with increased apoptosis in neurodegenerative conditions like Alzheimer's disease. Additionally in cancer interactions with proteins such as AKT suggest its potential role in cell proliferation and survival. Understanding its role could aid in the development of therapeutic interventions targeting mitochondrial dysfunction.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed : 11422376, PubMed : 1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
See full target information HSPD1

文献 (34)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed40999952

2025

HSPD1 regulates angiotensin II‑induced atrial fibrillation via the PPAR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yimeng Zhou,Lianzhi Zhang,Shunping Zhou,Wenjia Zhang,Qunlin Gong,Nan Xu,Jiahong Wang,Zhong Zhang,Nannan Chen

Acta pharmaceutica Sinica. B 15:3125-3148 PubMed40654366

2025

The crucial function of IDO1 in pulmonary fibrosis: From the perspective of mitochondrial fusion in lung fibroblasts and targeted molecular inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Shanchun Ge,Ye Zhang,Deqin Feng,Ting Zhu,Louqian Zhang,Chaofeng Zhang

CNS neuroscience & therapeutics 31:e70394 PubMed40237297

2025

Depletion of HSP60 in Microglia Leads to Synaptic Dysfunction and Depression-Like Behaviors Through Enhanced Synaptic Pruning in Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhui Zhu,Jinlong Chang,Liusuyan Tian,Xiuyan Yang,Weifen Li

Bone research 13:47 PubMed40229258

2025

SPI1 activates mitochondrial unfolded response signaling to inhibit chondrocyte senescence and relieves osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Zu,Shenghong Chen,Zhengyuan Li,Lin Hao,Wenhan Fu,Hui Zhang,Zongsheng Yin,Yin Wang,Jun Wang

Antioxidants (Basel, Switzerland) 14: PubMed40227213

2025

Large Fibrous Connective Tissue Reduces Oxidative Stress to Form a Living Cell Scaffold in Adipose Grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Yue,Zilong Cao,Tiran Zhang,Ningbei Yin,Liqiang Liu

International journal of molecular sciences 25: PubMed39684599

2024

Involvement of Melatonin, Oxidative Stress, and Inflammation in the Protective Mechanism of the Carotid Artery over the Torpor-Arousal Cycle of Ground Squirrels.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Hao,Yuting Han,Qi Zhao,Minghui Zhu,Xiaoxuan Liu,Yingyu Yang,Ning An,Dinglin He,Etienne Lefai,Kenneth B Storey,Hui Chang,Manjiang Xie

Communications biology 7:1229 PubMed39354016

2024

Maintaining mitochondrial DNA copy number mitigates ROS-induced oocyte decline and female reproductive aging.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyun Long,Yunchao Zheng,Xiaoling Deng,Jing Guo,Zhe Xu,Karin Scharffetter-Kochanek,Yanmei Dou,Min Jiang

Materials today. Bio 28:101235 PubMed39318374

2024

Decellularized adipose-derived matrix from Superficial layers of abdominal adipose tissue exhibits superior capacity of adipogenesis compared to deep layers.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomu Ma,Qiang Yue,Su Fu,Chunjun Liu,Jie Luan

Frontiers in oncology 14:1415762 PubMed39224812

2024

Clinicopathologic significance of heat shock protein 60 as a survival predictor in breast carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wang,Shengzhou Chen,Zhihong Wu,Jungang Ni

Theranostics 14:4278-4296 PubMed39113800

2024

Stimulation by exosomes from hypoxia-preconditioned hair follicle mesenchymal stem cells facilitates mitophagy by inhibiting the PI3K/AKT/mTOR signaling pathway to alleviate ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Li,Lei Zhao,Xinyu Geng,Jingyang Liu,Xu Zhang,Ying Hu,Jihan Qi,Hongliang Chen,Jiawei Qiu,Xiaoyu Zhang,Shizhu Jin
View all publications

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