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リコンビナントRabMAb

Anti-Hsp60 抗体 [EP1006Y] - Loading Control (ab45134)

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Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
  • Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
  • Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
  • Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1006Y] to Hsp60 - Loading Control
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Hsp60 antibody [EP1006Y] - Loading Control
    Hsp60 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1006Y] to Hsp60 - Loading Control

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human Hsp60 aa 400-500 (internal sequence).

  • ポジティブ･コントロール

    • T47D cell lysate, human breast carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab45134の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
特記事項
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

  • 関連疾患

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.

  • 配列類似性

    Belongs to the chaperonin (HSP60) family.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

画像

  • Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/20000 dilution

    Lane 1 : T47-D (Human mammary gland ductal carcinoma) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma) whole cell lysates
    Lane 3 : 293T (Human embryonic kidney) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 60 kDa



    Blocking/dilution buffer: 5% NFDM /TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

    Immunohistochemistry analysis of paraffin-embedded Human stomach labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the glandular epithelium of the Human stomach shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal heart lysates
    Lane 3 : Human fetal kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 60 kDa



    Blocking/dilution buffer: 5% NFDM /TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

    Immunohistochemistry analysis of paraffin-embedded Human transitional carcinoma of bladder labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on tumor cells of the Human bladder transitional cell carcinoma shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the epithelium of Mouse kidney tubules shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    Western blot - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    All lanes : Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134) at 1/50000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Rat brain lysates
    Lane 4 : Rat heart lysates
    Lane 5 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysates
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 7 : NIH/3T3 (Mouse embryo) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

    Immunohistochemistry analysis of paraffin-embedded Rat liver labeling Hsp60 with ab45134 at a 1/200 dilution. Cytoplasmic staining on the hepatocytes of the Rat liver shown. Secondary antibody ab97051 Goat Anti-Rabbit IgG H&L (HRP) used at a 1/500 dilution. Counter stained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab45134, secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)
    Anti-Hsp60 antibody [EP1006Y] - Loading Control (ab45134)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (16)

ab45134 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab45134 は 16 報の論文で使用されています。

  • Lim GY  et al. IFI16/Ifi202 released from breast cancer induces secretion of inflammatory cytokines from macrophages and promotes tumor growth. J Cell Physiol 238:1507-1519 (2023). PubMed: 37062941
  • Dörner K  et al. Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism. Nucleic Acids Res 50:2872-2888 (2022). PubMed: 35150276
  • Niikura R  et al. Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged AppNL-G-F/NL-G-F mice. Mol Brain 15:32 (2022). PubMed: 35387663
  • Li L  et al. Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis. Sci Rep 12:11830 (2022). PubMed: 35821263
  • Nebie O  et al. Human platelet lysate biotherapy for traumatic brain injury: preclinical assessment. Brain 144:3142-3158 (2021). PubMed: 34086871
View all Publications for this product

レビューと Q&A

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Western blot abreview for Anti-Hsp60 antibody [EP1006Y] - Loading Control

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human fibroblast cell and mitochondria fraction)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
Human fibroblast cell and mitochondria fraction
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Aug 09 2017

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