Anti-Hsp60 抗体 (ab82520)
Key features and details
- Goat polyclonal to Hsp60
- Suitable for: WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hsp60 antibody
Hsp60 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Hsp60 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Recombinant human Hsp60 protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82520の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
ターゲット情報
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機能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
関連疾患
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
配列類似性
Belongs to the chaperonin (HSP60) family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Omim: 118190 Human
- SwissProt: P10809 Human
- SwissProt: P63038 Mouse
- Unigene: 595053 Human
- Unigene: 727543 Human
- Unigene: 1777 Mouse
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別名
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
画像
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All lanes : Anti-Hsp60 antibody (ab82520) at 1/1000 dilution
Lane 1 : Hsp60
Active Recombinant Protein
Lane 2 : GroEL
Active Recombinant Protein
Lane 3 : Hsp65 Protein
Lane 4 : HeLa Cell Lysate
Lane 5 : 3T3 Cell Lysate
Lane 6 : RK-13 Cell Lysate
Predicted band size: 61 kDa
Observed band size: 61 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab82520 は 2 報の論文で使用されています。
- Calì T & Brini M Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells. Nat Protoc 16:5287-5308 (2021). PubMed: 34686857
- Cieri D et al. SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition. Cell Death Differ 25:1131-1145 (2018). PubMed: 29229997