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リコンビナント

Anti-Hsp60 抗体 [24/HSP60] (ab300659)

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Western blot - Anti-Hsp60 antibody [24/HSP60] (AB300659)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)
  • Anti-Hsp60 antibody [24/HSP60] (AB300659)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [24/HSP60] to Hsp60
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Hsp60 antibody [24/HSP60]
    Hsp60 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [24/HSP60] to Hsp60

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, WBmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human heart, mouse heart, rat heart tissue lysates; HeLa, MCF7, RAW264.7, NIH/3T3, C6 whole cell lysates. ICC/IF: HeLa; C6; RAW 264.7.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300659の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/250.
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
特記事項
ICC/IF
1/250.
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

  • 関連疾患

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.

  • 配列類似性

    Belongs to the chaperonin (HSP60) family.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

画像

  • Western blot - Anti-Hsp60 antibody [24/HSP60] (AB300659)
    Western blot - Anti-Hsp60 antibody [24/HSP60] (AB300659)
    All lanes : Anti-Hsp60 antibody [24/HSP60] (ab300659) at 1/1000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : HeLa (human epithelial cell line from cervical adenocarcinoma), whole cell lysate
    Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 7 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 8 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 15 seconds


    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)

    Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cells) labeling HSPD1 with ab300659 at 1/250 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing mitochondrial staining in HeLa cell line. The nuclear counter stain is DAPI (blue).

    ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used as a counterstain at 1/200 dilution along with ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) as the secondary used at 1/1000 labelling mitochondria (Red).

    Negative control 1 - ab150088 only (secondary only control) without counterstain primary

    Negative control 2 - ab150117 only (secondary only control) without target primary

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)

    Immunofluorescent analysis of 100% methanol fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cells) labeling HSPD1 with ab300659 at 1/250 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing mitochondrial staining in C6 cell line. The nuclear counter stain is DAPI (blue).

    ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used as a counterstain at 1/200 dilution along with ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) as the secondary used at 1/1000 labelling mitochondria (Red).

    Negative control 1 - ab150088 only (secondary only control) without counterstain primary

    Negative control 2 - ab150117 only (secondary only control) without target primary

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody [24/HSP60] (ab300659)

    Immunofluorescent analysis of 100% methanol fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophages) labeling HSPD1 with ab300659 at 1/250 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing mitochondrial staining in RAW 264.7 cell line. The nuclear counter stain is DAPI (blue).

    ab186735 Anti-TOMM20 Rabbit monoclonal antibody - Mitochondrial Marker was used as a counterstain at 1/200 dilution along with ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) as the secondary used at 1/1000 labelling mitochondria (Red).

    Negative control 1 - ab150088 only (secondary only control) without counterstain primary

    Negative control 2 - ab150117 only (secondary only control) without target primary

  • Anti-Hsp60 antibody [24/HSP60] (AB300659)
    Anti-Hsp60 antibody [24/HSP60] (AB300659)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300659 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300659 は論文での使用が確認できていません。

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