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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Hsp47 抗体 [EPR4217] (ab109117)

Reviews (8)Q&A (2)References (40)
Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)
  • Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
  • Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)
  • Anti-Hsp47 antibody [EPR4217] (ab109117)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4217] to Hsp47
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-Hsp47 antibody [EPR4217]
    Hsp47 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4217] to Hsp47

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, A431 and NIH/3T3 cell lysates; Human breast tissue; HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR4217

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109117の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (5)
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.

For unpurified use at 1/100.
IHC-P (1)
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.

For unpurified use at 1/100.
IHC-P
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

ターゲット情報

  • 機能

    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.

  • 関連疾患

    Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.

  • 配列類似性

    Belongs to the serpin family.

  • 細胞内局在

    Endoplasmic reticulum lumen.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 47 kDa heat shock protein antibody
    • 47 kDa heat shock protein precursor antibody
    • Arsenic transactivated protein 3 antibody
    • Arsenic-transactivated protein 3 antibody
    • AsTP 3 antibody
    • AsTP3 antibody
    • CBP 1 antibody
    • CBP 2 antibody
    • CBP1 antibody
    • CBP2 antibody
    • Cell proliferation-inducing gene 14 protein antibody
    • Collagen binding protein 1 antibody
    • Collagen binding protein 2 antibody
    • Collagen binding protein antibody
    • Collagen-binding protein 2 antibody
    • Collagen-binding protein antibody
    • Colligen antibody
    • Colligin 1 antibody
    • Colligin 2 antibody
    • Colligin antibody
    • colligin-1 antibody
    • colligin-2 antibody
    • gp46 antibody
    • Heat shock protein 47 antibody
    • Heat-shock protein 47 antibody
    • HGNC 1547 antibody
    • Hsp 47 antibody
    • J6 antibody
    • OI10 antibody
    • PIG 14 antibody
    • PIG14 antibody
    • PPROM antibody
    • Proliferation inducing gene 14 antibody
    • Proliferation inducing gene 14 protein antibody
    • RA A47 antibody
    • RA-A47 antibody
    • Rheumatoid arthritis antigen A 47 antibody
    • rheumatoid arthritis antigen A-47 antibody
    • Rheumatoid arthritis related antigen RA A47 antibody
    • Rheumatoid arthritis-related antigen RA-A47 antibody
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody
    • Serine or cysteine proteinase inhibitor clade H member 1 antibody
    • Serine or cysteine proteinase inhibitor clade H member 2 antibody
    • SERPH_HUMAN antibody
    • Serpin H1 antibody
    • Serpin peptidase inhibitor clade H member 1 antibody
    • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
    • Serpin peptidase inhibitor, clade H, member 1 antibody
    • SERPINH1 antibody
    • SERPINH2 antibody
    see all

画像

  • Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
    Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Hsp47 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109117 observed at 46 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109117 was shown to recognize Hsp47 in wild-type HAP1 cells as signal was lost at the expected MW in Hsp47 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsp47 knockout samples were subjected to SDS-PAGE. ab109117 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)

    ab109117 staining Hsp47 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

  • Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
    Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
    All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/2000 dilution

    Lane 1 : A431 Cell Lysate
    Lane 2 : NIH/3T3 Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 46 kDa

  • Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
    Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
    All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)

    ab109117, unpurified, at 1/100 dilution, staining Hsp47 in Human breast tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Hsp47 antibody [EPR4217] (ab109117)
    Anti-Hsp47 antibody [EPR4217] (ab109117)

参考文献 (40)

ab109117 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109117 は 40 報の論文で使用されています。

  • Murphy C  et al. Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation. ALTEX 40:141-159 (2023). PubMed: 35791294
  • Bons J  et al. Data-independent acquisition and quantification of extracellular matrix from human lung in chronic inflammation-associated carcinomas. Proteomics 23:e2200021 (2023). PubMed: 36228107
  • Peng X  et al. Prognostic significance and immune characteristics of APOE in gastric cancer. Aging (Albany NY) 15:13840-13853 (2023). PubMed: 38054821
  • Yu M  et al. Biglycan promotes hepatic fibrosis through activating heat shock protein 47. Liver Int 43:500-512 (2023). PubMed: 36371672
  • Wu M  et al. Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing. Pharmaceutics 14:N/A (2022). PubMed: 36297560
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 10 Abreviews or Q&A

Western blot abreview for Anti-Hsp47 antibody [EPR4217]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human primary fibroblasts (AG13334))
Gel Running Conditions
Reduced Denaturing (8% Bis-Tris)
Loading amount
10 µg
Specification
Human primary fibroblasts (AG13334)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Dec 20 2023

Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsp47 antibody [EPR4217]

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (chicken embryonic fibroblasts)
Permeabilization
Yes - 0.3% TritonX100
Specification
chicken embryonic fibroblasts
Blocking step
donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 May 27 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsp47 antibody [EPR4217]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fibroblasts)
Permeabilization
No
Specification
Fibroblasts
Blocking step
Zytomed Systems antibody diluent as blocking agent for 10 minute(s) · Concentration: 100%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Nov 24 2020

Western blot abreview for Anti-Hsp47 antibody [EPR4217]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Mar 15 2016

Western blot abreview for Anti-Hsp47 antibody [EPR4217]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 15 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Hsp47 antibody [EPR4217]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung tissue: fibroblasts)
Antigen retrieval step
None
Permeabilization
No
Specification
Lung tissue: fibroblasts
Blocking step
Zytomed Systems HRP-Polymer Kit Blocking Reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
HOPE-Fixation
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Apr 13 2015

Western blot abreview for Anti-Hsp47 antibody [EPR4217]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (chondrocyte cell line ATDC5)
Loading amount
30 µg
Specification
chondrocyte cell line ATDC5
Gel Running Conditions
Reduced Denaturing (4-20% Bis-Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Jun 12 2012

Western blot abreview for Anti-Hsp47 antibody [EPR4217]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hVSMCs)
Loading amount
5 µg
Specification
hVSMCs
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Aug 17 2011

Question

Can you tell me what is the concentration from lot YI081905CS?

Read More
Answer

The concentration for lot YI081905CS is 2.0130 mg/ml.

Read More

Question

Could you let me know the concentration of the antibody linked below? https://www.abcam.com/Hsp47-antibody-EPR4217-ab109117.html

Read More
Answer

Thank you for your enquiry.

I can confirm that ab109117anti-HSP47 antibody [EPR4217] is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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