Anti-Hsp47 抗体 [134CT7.1.8] (ab86750)
![Western blot - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)](https://www.abcam.co.jp/ps/products/86/ab86750/Images/ab86750-300884-anti-hsp47-antibody-134ct718-western-blot.jpg "Western blot - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)")
Key features and details
- Mouse monoclonal [134CT7.1.8] to Hsp47
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Hsp47 antibody [134CT7.1.8]
Hsp47 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [134CT7.1.8] to Hsp47 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra) -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Wild-type HAP1, HeLa, and HepG2 cell lysates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
134CT7.1.8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86750の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). |
ターゲット情報
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機能
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. -
関連疾患
Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass. -
配列類似性
Belongs to the serpin family. -
細胞内局在
Endoplasmic reticulum lumen. - Information by UniProt
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参照データベース
- Entrez Gene: 396228 Chicken
- Entrez Gene: 510850 Cow
- Entrez Gene: 485187 Dog
- Entrez Gene: 871 Human
- Entrez Gene: 12406 Mouse
- Entrez Gene: 29345 Rat
- Omim: 600943 Human
- SwissProt: P13731 Chicken
see all -
別名
- 47 kDa heat shock protein antibody
- 47 kDa heat shock protein precursor antibody
- Arsenic transactivated protein 3 antibody
see all
画像
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Western blot - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Hsp47 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab86750 observed at 46 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab86750 was shown to recognize Hsp47 in wild-type HAP1 cells as signal was lost at the expected MW in Hsp47 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsp47 knockout samples were subjected to SDS-PAGE. ab86750 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 5 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)](https://www.abcam.co.jp/ps/products/86/ab86750/Images/ab86750-228029-anti-hsp47-antibody-134ct718-western-blot.jpg)
Western blot - Anti-Hsp47 antibody [134CT7.1.8] (ab86750)All lanes : Anti-Hsp47 antibody [134CT7.1.8] (ab86750) at 5 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 4 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab86750 は論文での使用が確認できていません。