Anti-Hsp27 (phospho S86) 抗体

• ICC

Supplier Data

Immunocytochemistry - Anti-Hsp27 (phospho S86) antibody (AB17938)

HeLa cells stained for Hsp27 (green) using ab17938 at 1/250 dilution in ICC/IF. It was followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.

• WB

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Western blot - Anti-Hsp27 (phospho S86) antibody (AB17938)

Hsp25 (phospho S86) antibody image 6534.

Western blot using ab17938 on NIH3T3 cells treated with anisomycin.
Lane 1 : unstimulated cells
Lane 2 : cells stimulated with anisomycin
Lane 3 : cells stimulated with anisomycin. Antibody blocked with the non-phosphopeptide corresponding to the immunogen
Lane 4 : cells stimulated with anisomycin. Antibody blocked with generic phosphoserine-containing peptide
Lane 5 : cells stimulated with anisomycin. Antibody blocked with the phosphopeptide immunogen
Lane 6 : cells stimulated with anisomycin and treated with lambda phosphatase
10-30 μg of cell lysate can be loaded when using similar lysates with this antibody. Samples were run using SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with ab17938 for one hour at room temperature in 3% BSA-TBST buffer, following prior incubation with blocking

All lanes:

Western blot - Anti-Hsp27 (phospho S86) antibody (ab17938)

Predicted band size: 23 kDa

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