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Anti-HSF1 (phospho S326) 抗体 (ab115702)

Reviews (1)Q&A (2)References (10)
Western blot - Anti-HSF1 (phospho S326) antibody (ab115702)
  • Western blot - Anti-HSF1 (phospho S326) antibody (ab115702)

Key features and details

  • Rabbit polyclonal to HSF1 (phospho S326)
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-HSF1 (phospho S326) antibody
    HSF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to HSF1 (phospho S326)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Synthetic peptide corresponding to Human HSF1 (phospho S326).

  • ポジティブ･コントロール

    • HeLa whole cell lysate (ab150035); HeLa whole cell lysate (ab150035) Heat-Shocked;

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab115702の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 57 kDa). (ECL).
特記事項
WB
1/1000. Detects a band of approximately 85-95 kDa (predicted molecular weight: 57 kDa). (ECL).

ターゲット情報

  • 機能

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

  • 配列類似性

    Belongs to the HSF family.

  • ドメイン

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

  • 翻訳後修飾

    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.

  • 細胞内局在

    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Heat shock factor 1 antibody
      • Heat shock factor protein 1 antibody
      • Heat shock transcription factor 1 antibody
      • HSF 1 antibody
      • hsf1 antibody
      • HSF1_HUMAN antibody
      • HSTF 1 antibody
      • HSTF1 antibody
      see all

    画像

    • Western blot - Anti-HSF1 (phospho S326) antibody (ab115702)
      Western blot - Anti-HSF1 (phospho S326) antibody (ab115702)
      All lanes : Anti-HSF1 (phospho S326) antibody (ab115702) at 1/1000 dilution

      Lane 1 : MW marker
      Lane 2 : HeLa cell lysate
      Lane 3 : HeLa cell lysate Heat-Shocked (No recovery time)

      Predicted band size: 57 kDa



      ab115702 detects a band of 85-95kDa by Western blot depending on hyperphosphorylation of the native protein.
    • Western blot - Anti-HSF1 (phospho S326) antibody (ab115702)
      Western blot - Anti-HSF1 (phospho S326) antibody (ab115702)
      All lanes : Anti-HSF1 (phospho S326) antibody (ab115702) at 1/1000 dilution

      Lane 1 : MW markers
      Lane 2 : Recombinant HSF1
      Lane 3 : Recombinant HSF1 (phospho)

      Predicted band size: 57 kDa

    参考文献 (10)

    ab115702 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab115702 は 10 報の論文で使用されています。

    • Yang Z  et al. Crosstalk between heat shock factor 1 and signal transducer and activator of transcription 3 mediated by interleukin-8 autocrine signaling maintains the cancer stem cell phenotype in liver cancer. J Gastroenterol Hepatol 38:138-152 (2023). PubMed: 36300571
    • Fujimoto M  et al. HSF1 phosphorylation establishes an active chromatin state via the TRRAP-TIP60 complex and promotes tumorigenesis. Nat Commun 13:4355 (2022). PubMed: 35906200
    • Zhang Z  et al. Heat Shock Protein 70 Mediates the Protective Effect of Naringenin on High-Glucose-Induced Alterations of Endothelial Function. Int J Endocrinol 2022:7275765 (2022). PubMed: 35958293
    • Moreno R  et al. The stress-responsive kinase DYRK2 activates heat shock factor 1 promoting resistance to proteotoxic stress. Cell Death Differ 28:1563-1578 (2021). PubMed: 33268814
    • Trivedi R  et al. Augmentation of the heat shock axis during exceptional longevity in Ames dwarf mice. Geroscience 43:1921-1934 (2021). PubMed: 33846884
    • Jurivich DA  et al. Multifactorial Attenuation of the Murine Heat Shock Response With Age. J Gerontol A Biol Sci Med Sci 75:1846-1852 (2020). PubMed: 31612204
    • Pan X  et al. Heat shock factor 1 suppresses the HIV-induced inflammatory response by inhibiting nuclear factor-?B. Cell Immunol 327:26-35 (2018). PubMed: 29525181
    • Jin X  et al. Modulation of Heat Shock Factor 1 Activity through Silencing of Ser303/Ser307 Phosphorylation Supports a Metabolic Program Leading to Age-Related Obesity and Insulin Resistance. Mol Cell Biol 38:N/A (2018). PubMed: 29941492
    • Jin X  et al. WITHDRAWN: Abrogation of heat shock factor 1 (Hsf1) phosphorylation deregulates its activity and lowers activation threshold, leading to obesity in mice. J Biol Chem N/A:N/A (2017). PubMed: 28724629
    • Lee JH  et al. Mitochondrial Respiratory Dysfunction Induces Claudin-1 Expression via Reactive Oxygen Species-mediated Heat Shock Factor 1 Activation, Leading to Hepatoma Cell Invasiveness. J Biol Chem 290:21421-31 (2015). PubMed: 26157141

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-HSF1 (phospho S326) antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    30 µg
    Specification
    Mouse embryonic stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 17 2020

    Question

    immunogen for ab115702 so that we can do an alignment with bovine

    Read More
    Answer

    J’ai contacté le laboratoire pour savoir si l’un de nos anti- HSF1 (phospho S326) anticorps fonctionnerait chez la vache. Malheureusement, nous n’avons que trois anticorps : ab76076, ab1050 et ab115702 au catalogue et ils ont une homologie de 64%, 77% et 77% respectivement avec la séquence bovine. Ceci indique qu’il y’a des grandes chances que ces anticorps ne fonctionneront pas chez la vache. Si vous souhaitez quand même tester l'un de ces anticorps, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de 'anticorps pourra être utilisable lors d’une prochaine commande.
    Etapes à suivre :
    1. Nous confirmer que vous souhaiteriez tester l'anticorps afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de l'anticorps
    2. Acheter l'anticorps par téléphone, fax ou internet (www.abcam.com)
    3. Le tester chez la vache
    4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
    5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre produit abcam. Même si l'anticorps est démontré ne pas fonctionner chez la vache, après soumission de votre Abreview, vous recevrez quand même une remise sur votre prochaine commande.
    N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.
    Termes et Conditions : www.abcam.com/collaborationdiscount.

    Read More

    Question

    Je voudrais savoir les conditions et les prix pour la préparation d'anticorps spécifiques.

    Read More
    Answer

    Merci de nous avoir contactés.
    Nous sommes heureux d’avoir la possibilité de vous proposer un service par Epitomics, une société Abcam qui peut vous préparer des anticorps spécifiques.
    Pour plus d'informations, visitez: http://www.epitomics.com/services/
    e-mail: service@epitomics.com
    Sinon, contactez-Li Li chez Epitomics: li.li@epitomics.com
    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.
    Monoclonal de lapin gratuit pour tout achat d'anticorps primaire, dans la limite des stocks disponibles. Notez le code "RABMAB-XBSMG" sur votre prochaine commande d’anticorps primaire. Pour plus d'informations, visitez le lien suivant: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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