Anti-hSET1/SET1 抗体 (ab70378)
Key features and details
- Rabbit polyclonal to hSET1/SET1
- Suitable for: ChIP, IHC-P, ChIP/Chip, WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-hSET1/SET1 antibody
hSET1/SET1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to hSET1/SET1 -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, IHC-P, ChIP/Chip, WB, IP, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human hSET1/SET1.
Database link: O15047 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70378の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
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| IHC-P | (1) |
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ChIP/Chip |
Use 10 µg for µg of chromatin.
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| WB | (1) |
1/10000 - 1/25000. Detects a band of approximately 260-280 kDa (predicted molecular weight: 186 kDa).
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| IP |
Use at 2-10 µg/mg of lysate.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
Use with paraformaldehyde or methanol-fixed cells. |
| 特記事項 |
|---|
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ChIP
Use at an assay dependent concentration. |
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IHC-P
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ChIP/Chip
Use 10 µg for µg of chromatin. |
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WB
1/10000 - 1/25000. Detects a band of approximately 260-280 kDa (predicted molecular weight: 186 kDa). |
|
IP
Use at 2-10 µg/mg of lysate. |
|
ICC/IF
Use at an assay dependent concentration. Use with paraformaldehyde or methanol-fixed cells. |
ターゲット情報
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機能
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. -
配列類似性
Contains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain. -
細胞内局在
Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes. - Information by UniProt
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参照データベース
- Entrez Gene: 782887 Cow
- Entrez Gene: 9739 Human
- Entrez Gene: 233904 Mouse
- Omim: 611052 Human
- SwissProt: O15047 Human
- Unigene: 297483 Human
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別名
- Histone-lysine N-methyltransferase SETD1A antibody
- hSET1A antibody
- KMT2F antibody
see all
画像
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Western blot - Anti-hSET1/SET1 antibody (ab70378)All lanes : Anti-hSET1/SET1 antibody (ab70378) at 1/25000 dilution
Lane 1 : HEK293T Whole cell lysate
Lane 2 : HeLa Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lane 4 : Hep-G2 Whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 186 kDacells prepared using NETN lysis buffer
Detection: Chemiluminescence with an exposure time of 75 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSET1/SET1 antibody (ab70378)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1/SET1 using ab70378 at 1/5000 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB.
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Immunoprecipitation - Anti-hSET1/SET1 antibody (ab70378)hSET1/SET1 was immunoprecipitated from 293T nuclear extracts with 6 µg/mg lysate ab70378. Western blot was performed from the immunoprecipitate using ab70378 at 1/1000 dilution.
Lane 1: ab70378 IP in 293T nuclear extract.
Lane 2: Control IgG IP in 293T nuclear extract.
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ChIP on chip - Anti-hSET1/SET1 antibody (ab70378)Ab70378 (10 µg) was used to immunoprecipitate chromatin from K562 cells. Immunoprecipitated and reference DNA were amplified by ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray and analyzed. As a control a similar experiment was performed using normal rabbit IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab70378 は 18 報の論文で使用されています。
- Chen R et al. Cell type-specific mechanism of Setd1a heterozygosity in schizophrenia pathogenesis. Sci Adv 8:eabm1077 (2022). PubMed: 35245111
- Garipler G et al. The BTB transcription factors ZBTB11 and ZFP131 maintain pluripotency by repressing pro-differentiation genes. Cell Rep 38:110524 (2022). PubMed: 35294876
- Chen J et al. Anlotinib suppresses MLL-rearranged acute myeloid leukemia cell growth by inhibiting SETD1A/AKT-mediated DNA damage response. Am J Transl Res 13:1494-1504 (2021). PubMed: 33841673
- Deng X et al. Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1. Invest Ophthalmol Vis Sci 62:1 (2021). PubMed: 33938913
- Francis M et al. SETD1 and NF-κB Regulate Periodontal Inflammation through H3K4 Trimethylation. J Dent Res 99:1486-1493 (2020). PubMed: 32762504