Anti-HSDL2 抗体 [EPR13876(B)] (ab181174)
![Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.co.jp/ps/products/181/ab181174/Images/ab181174-214653-anti-hsdl2-antibody-epr13876b-western-blot.jpg "Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13876(B)] to HSDL2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HSDL2 antibody [EPR13876(B)]
HSDL2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13876(B)] to HSDL2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, 293 and Human fetal liver lysates, Human kidney tissue, HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13876(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181174の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Has apparently no steroid dehydrogenase activity. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 SCP2 domain. -
細胞内局在
Peroxisome. - Information by UniProt
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参照データベース
- Entrez Gene: 84263 Human
- SwissProt: Q6YN16 Human
- Unigene: 59486 Human
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別名
- C9orf99 antibody
- FLJ25855 antibody
- hsdl2 antibody
see all
画像
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Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)All lanes : Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.co.jp/ps/products/181/ab181174/Images/ab181174-214656-anti-hsdl2-antibody-epr13876b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)Immunofluorescent staining of -20℃ Acetone fixed HeLa cells labeling HSDL2 using ab181174 at a 1/100 dilution and a Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.co.jp/ps/products/181/ab181174/Images/ab181174-214655-anti-hsdl2-antibody-epr13876b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling HSDL2 using ab181174 at a 1/100 dilution using prediluted HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.co.jp/ps/products/181/ab181174/Images/ab181174-214654-anti-hsdl2-antibody-epr13876b-western-blot.jpg)
Western blot - Anti-HSDL2 antibody [EPR13876(B)] (ab181174)Anti-HSDL2 antibody [EPR13876(B)] (ab181174) at 1/10000 dilution + Human fetal liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 45 kDa -
![Anti-HSDL2 antibody [EPR13876(B)] (ab181174)](https://www.abcam.co.jp/ps/products/181/ab181174/Images/ab181174-5-benefits-of-recombinant-antibodies.png)
Anti-HSDL2 antibody [EPR13876(B)] (ab181174)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab181174 は 1 報の論文で使用されています。
- Sun Q et al. Role of Hydroxysteroid Dehydrogenase-Like 2 (HSDL2) in Human Ovarian Cancer. Med Sci Monit 24:3997-4008 (2018). PubMed: 29894468