Anti-HSD3B1 抗体 [EPR9687] (ab167417)
![Western blot - Anti-HSD3B1 antibody [EPR9687] (ab167417)](https://www.abcam.co.jp/ps/products/167/ab167417/Images/ab167417-165890-anti-hsd3b1-antibody-epr9687-western-blot.jpg "Western blot - Anti-HSD3B1 antibody [EPR9687] (ab167417)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9687] to HSD3B1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HSD3B1 antibody [EPR9687]
HSD3B1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9687] to HSD3B1 -
由来種
Rabbit -
特異性
The immunogen used for this product shares 83% homology and 6 continuous identical amino acids with HSD3B2(SwissProt: P26439). Cross-reactivity with this protein has not been confirmed experimentally.
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アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human placenta cell lysate, Caco-2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR9687 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167417の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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| ICC/IF |
1/250 - 1/500.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
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ICC/IF
1/250 - 1/500. |
追加情報
Is unsuitable for IHC-P or IP.
ターゲット情報
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機能
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. -
組織特異性
Placenta and skin. Predominantly expressed in mammary gland tissue. -
パスウェイ
Lipid metabolism; steroid biosynthesis. -
配列類似性
Belongs to the 3-beta-HSD family. -
細胞内局在
Endoplasmic reticulum membrane. Mitochondrion membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3283 Human
- Entrez Gene: 3284 Human
- Omim: 109715 Human
- SwissProt: P14060 Human
- SwissProt: P26439 Human
- Unigene: 364941 Human
- Unigene: 654399 Human
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別名
- 3 beta and steroid delta isomerase 1 antibody
- 3 beta and steroid hydroxy delta 5 steroid dehydrogenase antibody
- 3 beta HSD I antibody
see all
画像
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Western blot - Anti-HSD3B1 antibody [EPR9687] (ab167417)Anti-HSD3B1 antibody [EPR9687] (ab167417) at 1/1000 dilution + Human placenta cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 42 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-HSD3B1 antibody [EPR9687] (ab167417)](https://www.abcam.co.jp/ps/products/167/ab167417/Images/ab167417-165889-anti-hsd3b1-antibody-epr9687-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSD3B1 antibody [EPR9687] (ab167417)Immunofluorescent analysis of Caco-2 cells labeling HSD3B1 with ab167417 at 1/250 dilution. -
![Anti-HSD3B1 antibody [EPR9687] (ab167417)](https://www.abcam.co.jp/ps/products/167/ab167417/Images/ab167417-3-benefits-of-recombinant-antibodies.png)
Anti-HSD3B1 antibody [EPR9687] (ab167417)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab167417 は 4 報の論文で使用されています。
- Fraichard C et al. Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function. Int J Mol Sci 22:N/A (2021). PubMed: 33445576
- E Silva PV et al. Effects of isolated or combined exposure to sibutramine and rosuvastatin on reproductive parameters of adult male rats. J Appl Toxicol 40:947-964 (2020). PubMed: 32072669
- Nedder M et al. Uptake of Cerium Dioxide Nanoparticles and Impact on Viability, Differentiation and Functions of Primary Trophoblast Cells from Human Placenta. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32635405
- Fraichard C et al. Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation. Mol Cell Endocrinol 499:110586 (2020). PubMed: 31539598