Anti-Hsc70 抗体 (ab154415)
Key features and details
- Rabbit polyclonal to Hsc70
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hsc70 antibody
Hsc70 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hsc70 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Xenopus laevis, Zebrafish
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免疫原
Recombinant protein fragment, corresponding to a region within amino acids 470-635 of Human Hsc70 (Uniprot ID: P11142).
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ポジティブ･コントロール
- Recombinant human Hsc70 protein (ab78431) can be used as a positive control in WB. 293T, A431, H1299, HepG2, Molt-4 and Raji cell lysates; Mouse brain whole cell lysate; HeLa cells; Human U87 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154415の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 70 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the heat shock protein 70 family. -
ドメイン
The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
細胞内局在
Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 395853 Chicken
- Entrez Gene: 281831 Cow
- Entrez Gene: 3312 Human
- Entrez Gene: 15481 Mouse
- Entrez Gene: 24468 Rat
- Entrez Gene: 573376 Zebrafish
- Omim: 600816 Human
- SwissProt: O73885 Chicken
see all -
別名
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
- Epididymis luminal protein 33 antibody
see all
画像
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Western blot - Anti-Hsc70 antibody (ab154415)Anti-Hsc70 antibody (ab154415) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody (ab154415)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Hsc70 using ab154415 at 1/200 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab154415)Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue, labeling Hsc70 using ab154415 at 1/100 dilution. -
Western blot - Anti-Hsc70 antibody (ab154415)Anti-Hsc70 antibody (ab154415) at 1/10000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154415 は 1 報の論文で使用されています。
- Zurawski Z et al. Disabling the Gß?-SNARE interaction disrupts GPCR-mediated presynaptic inhibition, leading to physiological and behavioral phenotypes. Sci Signal 12:N/A (2019). PubMed: 30783011