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HRP Anti-VSV-G tag 抗体 (ab3556)

Submit a review Q&A (2)References (4)

Key features and details

  • HRP Rabbit polyclonal to VSV-G tag
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

製品の概要

  • 製品名

    HRP Anti-VSV-G tag antibody
    VSV-G tag 一次抗体 製品一覧

  • 製品の詳細

    HRP Rabbit polyclonal to VSV-G tag

  • 由来種

    Rabbit

  • 標識

    HRP

  • アプリケーション

    適用あり: WB, ELISAmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide corresponding to VSV-G tag conjugated to keyhole limpet haemocyanin.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3556の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000.
ELISA
1/5000 - 1/20000.
特記事項
WB
1/1000 - 1/5000.
ELISA
1/5000 - 1/20000.

ターゲット情報

  • 関連性

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.

  • 別名

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

参考文献 (4)

ab3556 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3556 は 4 報の論文で使用されています。

  • Zhang X  et al. microRNA-induced translational control of antiviral immunity by the cap-binding protein 4EHP. Mol Cell 81:1187-1199.e5 (2021). PubMed: 33581076
  • Masuda Y  et al. Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathway. Nucleic Acids Res 46:11340-11356 (2018). PubMed: 30335157
  • Bellon A  et al. VEGFR2 (KDR/Flk1) signaling mediates axon growth in response to semaphorin 3E in the developing brain. Neuron 66:205-19 (2010). WB . PubMed: 20434998
  • Thyrell L  et al. Interferon alpha-induced apoptosis in tumor cells is mediated through the phosphoinositide 3-kinase/mammalian target of rapamycin signaling pathway. J Biol Chem 279:24152-62 (2004). PubMed: 15056668

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Hello,



I am looking for an antibody for detecting whether my cells are expressing VSV-G (Western blot). I have found several ab on-line, but do you also have cell lysate as a positive control?



Best regards

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Answer

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

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Question

WESTERN BLOT TECHNICAL ENQUIRY - Wed 11 Feb 2004, 10:42hrs ========================================================== ANTIBODY CODE ab3556 BATCH NUMBER 37614 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific band I immunoprecipitate 2 VSV-G tagged proteins using a mouse monoclonal antibody and want to detect my immunoprecipitatd proteins with the VSV G hrp rabbit polyclonal antibody. I have no problem to detect the first one (so I know that my immunoprecipitation is OK), but the problem is that at the size of my second VSV-G tagged proteins there are plenty of non specific bands, so I detect a band even in my controls where I don't express this second protein. So I cannot prove that I have immunoprecipitated my second protein too. I tried different dilutions of the antibody but this does not solve my problem. Can you indicate me your Western Blot conditions? Furthermore, this antibody reveals the heavy and light chains. I wanted to know if this antody is known to cross-react ? Thank you Nathalie Franck SAMPLE HeLa cell extract incubated with a VSV-G monoclonal antibody (which works well) PRIMARY ANTIBODY VSV-g hrp tested at different dilutions 1/1000-1/5000 SECONDARY ANTIBODY none DETECTION METHOD super signal (Pierce) POSITIVE AND NEGATIVE CONTROLS USED Negative control: Lysates transfected with a control protein also VSV-G tagged. I detect this protein (no contaminating bands at this size (100 kDa). But at the size of my protein of interest, I detect a contaminating band in my negative control ANTIBODY STORAGE CONDITIONS Storage: 4°C SAMPLE PREPARATION Buffer containing 150 mM NaCL, Tris 10 mM, NP40 0,5%, Triton-X-100 1%, EDTA, EGTA, Na3VO4, PMSF, protease inhibitor (Roche) AMOUNT OF PROTEIN LOADED 15-20 µg of total cell lysate or 10 µl of immunoprecipitation product (I use 500 µg for one immunoprecipitation; beads are respuspended in 25 µl of SB2X ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 12,5% TRANSFER AND BLOCKING CONDITIONS Transfer 45 minutes 300 mA. I block my membran with 5% dry non fat milk in Tris buffered saline containing 0,1% Tween. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Dilution of the antibody and incubation time (overnight or 1h at ambiant temperature

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Answer

Thank you very much for your patience. I am working with the originator of ab3556 to help figure out what may be going on with this particular antibody. We have a few more questions however that will help us out. 1) What exactly does your negative control consist of? 2) How are you isolating the monoclonal antibody/VSV-g tagged protein? 3) Do you isolate by eluting the protein or centrifuging? 4) How do you know it is recognizing the heavy and light chains? 5)Have you tried any inhibition studies?

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